The effect of ultrasonic-assisted enzymes extraction on antioxidant polysaccharide activity in dandelion

文献类型： 外文期刊

第一作者： Ni, Shuang

作者： Ni, Shuang;Zhao, Haolan;Yang, Shaohua;Cui, Kai

作者机构：

关键词： Dandelion; Polysaccharides; UAEE; Component; Antioxidant activity

期刊名称：ULTRASONICS SONOCHEMISTRY （ 影响因子：9.7； 五年影响因子：9.1 ）

ISSN： 1350-4177

年卷期： 2025 年 116 卷

页码：

收录情况： SCI

摘要： In this study, ultrasonic-assisted enzymatic extraction (UAEE) method was used to obtain the small molecule dandelion polysaccharides. Based on single-factor experiments, the process of extraction the extraction conditions were further optimized by response surface method (RSM) combined with the Box-Behnken design (BBD). The results showed that in combination with enzymes, with a ratio of 1:32 (g/ml), an enzymolysis temperature 55 degrees C, an ultrasonic temperature 75 degrees C and the ultrasonic time 55 min. The maximum extraction yield of crude polysaccharides was 3.127 %. After purification by DEAE-cellulose chromatography and Sephadex G-75 gel filtration, a novel polysaccharide (DANP-II) was extracted and the results of high performance liquid chromatography (HPLC) indicated its average molecular weight was 3.373 kDa. Gas chromatography (GC) analysis showed that DANP-II was mainly contained by glucose and little of glucuronic acid, mannose, galactose, and arabinose. In vitro antioxidant experiments have demonstrated that DANP-II can alleviate H2O2-induced cellular damage, reduce apoptosis rates, and exhibit robust antioxidant activity. Hence, DANP-II can be utilized as a natural antioxidant to enhance protection against oxidative stress, providing theoretical guidance for the utilization of low-molecular-weight dandelion polysaccharides.

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