Identification of insect sources of honey in China based on real-time fluorescent LAMP technology

文献类型： 外文期刊

第一作者： Gao, Jie

作者： Gao, Jie;Jin, Xiue;Gong, Bo;Li, Jingjing;Wang, Jun;Gao, Jie;Li, Jingjing;Gao, Jie;Chen, Ailiang;Tan, Jianxin;Wang, Jun

作者机构：

关键词： Apis mellifera; Apis cerana; Honey; Loop -mediated isothermal amplification; (LAMP); Major Royal Jelly Protein 2 ( MRJP2 )

期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.52； 五年影响因子：4.942 ）

ISSN： 0889-1575

年卷期： 2023 年 115 卷

页码：

收录情况： SCI

摘要： The main insect sources of Chinese honey are Apis cerana and Apis mellifera. A. cerana honey has a higher nutritional value; therefore, its price is invariably several times higher than that of A. mellifera honey. Driven by economic interests, low-priced A. mellifera honey is often misrepresented as the more valuable A. cerana honey. In this study, Apis Major Royal Jelly Protein 2 (MRJP2) was used as the target gene to design specific primers for two bee species, a rapid real-time fluorescent LAMP method was established, and the specificity and sensitivity of this method were analyzed. Results showed that this method could effectively distinguish between the A. cerana and A. mellifera honey. The detection sensitivity of the A. cerana honey and A. mellifera honey primers were 4 ng/ mu L and 1 ng/mu L, respectively. Eight commercially available honey samples were tested with the method devel-oped in this study, and four adulterated honey samples were identified. The detection method proposed in this study may improve the quality of and safety measures for honey.

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