Isolation and identification of Bacillus and abilities of 3 functional strains to control powdery mildew and promote seedling growth of Malus sieversii

文献类型： 外文期刊

第一作者： Liu, Bin

作者： Liu, Bin;Ji, Shida;Zhang, Huifang;Han, Jing;Qi, Jinyu;Liu, Zhihua;Xu, Yanrong;Zhang, Ping;Fan, Haijuan;Wang, Jinjie;Xu, Yanrong;Ma, Yue

作者机构：

关键词： Bacillus spp; Isolation; Identification; Malus sieversii; Powdery mildew disease; Biocontrol

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.8； 五年影响因子：2.0 ）

ISSN： 0929-1873

年卷期： 2023 年 167 卷 1 期

页码：

收录情况： SCI

摘要： To effectively control the powdery mildew (Podosphaera leucotricha) of Malus sieversii (Msi) and promote the growth of Msi seedlings, 170 Bacillus strains (NECC10001-NECC10170) were isolated from 10 Msi tree rhizosphere soils from Xinjiang wild fruit forest, China; 55 Bacillus isolates with typical representative characteristics were identified, including 10 Bacillus species. Owing to their rapid growth and good biocontrol effects, B. velezensis NECC10057 (Bve057), B. amyloliquefaciens NECC10112 (Bam112), and B. subtilis NECC10145 (Bsu145) were selected for further study. Bve057, Bam112, and Bsu145 significantly increased the number of lateral roots and biomass of Msi seedlings. Plant height, fresh weight, and dry weight of Msi treated with Bve057, Bam112, and Bsu145 fermentation broths for 30 d was at least 25%, 11%, and 50% higher, respectively, than that of the control. Treatment with fermentation broths of the three Bacillus strains increased the number of lateral roots, which enhanced water and nutrient absorption and promoted Msi seedling growth. After treatment with Bve057, Bam112, and Bsu145 fermentation broth for 6 d, catalase activity of Msi seedlings reached a peak (> 812.83 mu mol/min/g), which was 295% higher than that in the control. Furthermore, the hydrogen peroxide and malondialdehyde content of treated Msi leaves was significantly lower than that of the control. Notably, leaves treated with Bacillus showed open stomata, which improves the photosynthetic rate and protects leaf tissue integrity. Thus, the three Bacillus isolates endow Msi with the ability to resist P. leucotricha by increasing the antioxidant capacity and photosynthetic rate. Thus, Bve057, Bam112, and Bsu145 isolated from Msi tree rhizosphere soils from Xinjiang wild fruit forest promoted Msi growth and enhanced its resistance against powdery mildew.

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