Genome Mining and Analysis of PKS Genes in Eurotium cristatum E1 Isolated from Fuzhuan Brick Tea

文献类型： 外文期刊

第一作者： Guo, Xiaoxiao

作者： Guo, Xiaoxiao;Chen, Fusheng;Shao, Yanchun;Wang, Xiaohong;Guo, Xiaoxiao;Chen, Fusheng;Shao, Yanchun;Wang, Xiaohong;Liu, Jiao;Zhou, Youxiang;Liu, Jiao;Zhou, Youxiang

作者机构：

关键词： Eurotium cristatum; Fuzhuan brick tea; whole genome; polyketide synthase; secondary metabolites; emodin; flavoglaucin

期刊名称：JOURNAL OF FUNGI （ 影响因子：5.724； 五年影响因子：6.413 ）

ISSN：

年卷期： 2022 年 8 卷 2 期

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收录情况： SCI

摘要： Eurotium cristatum as the dominant fungi species of Fuzhuan brick tea in China, can produce multitudinous secondary metabolites (SMs) with various bioactivities. Polyketides are a very important class of SMs found in E. cristatum and have gained extensive attention in recent years due to their remarkable diversity of structures and multiple functions. Therefore, it is necessary to explore the polyketides produced by E. cristatum at the genomic level to enhance its application value. In this paper, 12 polyketide synthase (PKS) genes were found in the whole genome of E. cristatum E1 isolated from Fuzhuan brick tea. In addition, the qRT-PCR results further demonstrated that these genes were expressed. Moreover, metabolic analysis demonstrated E. cristatum E1 can produce a variety of polyketides, including citreorosein, emodin, physcion, isoaspergin, dihydroauroglaucin, iso-dihydroauroglaucin, aspergin, flavoglaucin and auroglaucin. Furthermore, based on genomic analysis, the putative secondary metabolites clusters for emodin and flavoglaucin were proposed. The results reported here will lay a good basis for systematically mining SMs resources of E. cristatum and broadening its application fields.

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