A polysaccharide from Lycium barbarum L.: Structure and protective effects against oxidative stress and high-glucose-induced apoptosis in ARPE-19 cells
文献类型： 外文期刊
第一作者： Liu, Jianfei
作者： Liu, Jianfei;Li, Yunchun;Qiu, Hongdeng;Di, Duolong;Liu, Jianfei;Li, Yunchun;Qiu, Hongdeng;Di, Duolong;Liu, Jianfei;Qiu, Hongdeng;Di, Duolong;Pu, Qiaosheng;Cao, Youlong
作者机构：
关键词： Lycium barbarum polysaccharides; Structural characterization; ARPE-19 cells
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.025； 五年影响因子：7.626 ）
ISSN： 0141-8130
年卷期： 2022 年 201 卷
页码：
收录情况： SCI
摘要： Lycium barbarum polysaccharides (LBPs) are beneficial for vision; however, relevant research has mainly focused on entire crude polysaccharides, with the basis and exact structure of the polysaccharide rarely explored. In this study, LICP009-3F-2a, a novel polysaccharide from Lycium barbarum L., was separated and then purified using anion-exchange and size-exclusion chromatography. Structural characteristics were investigated using chemical and spectroscopic methods, which revealed that LICP009-3F-2a has an Mw of 13720 Da and is an acidic heteropolysaccharide composed of rhamnose (39.1%), arabinose (7.4%), galactose (22.5%), glucose (8.3%), galacturonic acid (13.7%), and glucuronic acid (4.0%). Linkage and NMR data revealed that LICP009-3F-2a has the following backbone: -> 2)-alpha-L-Rha-(1 -> 2,4)-alpha-L-Rha-(1 -> 4)-alpha-D-GalAp-(1 -> 3,6)-beta-D-Galp-(1 -> 3,6)-beta-D-Galp-(1 -> 6)-beta-D-Galp-(1 ->, with three main branches, including: alpha-L-Araf-(1 -> 5)-alpha-L-Araf-(1 -> 6)-beta-D-Glcp-(1 -> 2,4)-alpha-L-Rha-(1 ->, beta-D-Glcp-(1 -> 4)-beta-D-Glcp-(1 -> 3,6)-beta-D-Galp-(1 ->, and beta-D-Galp-(1 -> 3)-beta-D-Galp-(1 -> 3,6)-beta-D-Galp-(1 ->. Differential scanning colorimetry and thermogravimetric analysis showed that LICP009-3F-2a is thermally stable, while X-ray diffractometry showed that LICP009-3F-2a has a semi-crystalline structure. In addition, LICP009-3F-2a protects ARPE-19 cells from H2O2-induced oxidative damage by regulating the expression of antioxidant SOD1 and CAT enzymes and down-regulating MMP2 expression. Moreover, LICP009-3F-2a promotes the proliferation of ARPE-19 cells in a concentration-dependent manner, and protects ARPE-19 cells from hyperglycemia by inhibiting apoptosis.
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