Comprehensive evaluation of novel fungicide benzovindiflupyr at the enantiomeric level: Bioactivity, toxicity, mechanism, and dissipation behavior

文献类型： 外文期刊

第一作者： An, Xiaokang

作者： An, Xiaokang;Pan, Xinglu;Li, Runan;Dong, Fengshou;Xu, Jun;Wu, Xiaohu;An, Xiaokang;Zheng, Yongquan;An, Xiaokang;Zhu, Wentao;Dong, Fengshou

作者机构：

关键词： Enantioseparation; Absolute configuration; Enantioselective difference; Molecular docking; Mechanism

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：9.8； 五年影响因子：9.6 ）

ISSN： 0048-9697

年卷期： 2023 年 860 卷

页码：

收录情况： SCI

摘要： Racemates in the environment can lead to inaccurate risk assessment. To obtain the enantiomeric level information of benzovindiflupyr for accurate risk assessment, the absolute configuration of benzovindiflupyr was first confirmed, and the enantioseparation method was developed by supercritical fluid chromatography tandem mass spectrometry. The enantioselectivity for bioactivity and toxicity was investigated, and the mechanism was explored by molecular docking and detecting succinate dehydrogenase (SDH) activity and content of succinate acid. 1S,4R-(-)-benzovindiflupyr was identified as the most active against the six targeted phytopathogens, which showed higher 1.7-54.5 times than 1R,4S(+)-benzovindiflupyr. Additionally, 1S,4R-(-)-benzovindiflupyr (LD50: 21.54 mu g L-1) was 103.7 times more toxic than 1R,4S-(+)-benzovindiflupyr against Daphnia magna. 1S,4R-(-)-benzovindiflupyr had a stronger affinity for SDH and significantly inhibited SDH activity, resulting in an increase in succinate acid in the tricarboxylic acid cycle, while its downstream products, fumaric and L-malic acid were significantly reduced. Moreover, the dissipation behavior of benzovindiflupyr on three vegetables was evaluated. 1S,4R-(-)-benzovindiflupyr was preferentially degraded in tomato, but opposite in leaves. The enantioselectivity in pepper and cucumber leaves was the same as in tomato, while there was no enantioselectivity in pepper and cucumber. The study provides a basis for accurate risk assessment and the development of high-effective and low-risk fungicides.

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