Cloning and characterization of a novel avirulence gene (arp3) from Xanthomonas oryzae pv. oryzae

文献类型： 外文期刊

第一作者： Liang, B

作者： Liang, B;Yu, TG;Guo, B;Yang, C;Dai, LY;Shen, DL

作者机构：

关键词： Xanthomonse oryzae pv. oryzae;Apr3(avrBs3-related protein 3);Avr Bs3 gene family;TRANSCRIPTIONAL ACTIVATION DOMAIN;III SECRETION;PLANT-CELL;C-TERMINUS;PROTEIN;BACTERIAL;AVRBS3;TRANSLOCATION;SIGNALS;YOPE

期刊名称：DNA SEQUENCE （ 影响因子：0.408； 五年影响因子：0.594 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A novel avirulence gene was cloned from Xanthomonas oryzae pv. oryzae strain PXO339 , which is the standard representative of the Philippines race 9a. The full-length gene spans 2118 bp and encodes a protein of 705 amino acids. BLAST search in NCBI indicated that the gene belongs to avrBs3 gene family, and designated arp3 (AvrBs3-related protein 3, arp3). The central region of the arp3 contains only 5.5 copies of 102 bp repeats, the smallest copy number of repeats found in avrBs3 gene family by now. Together with the repeats is heptad repeats, resembling leucine zippers. Three functional nuclear localization signals and an acidic activation domain are also found in the C-terminal region. However, the arp3 lacks of two segments in its N-terminal region, which is unique in avrBs3 gene family. Southern blotting data showed that the arp3 is present as a single-copy in genomic DNA of PXO339 and locus in plasmid clone. The arp3 could be expressed in vitro in Escherichia coli BL21 and a 128 kDa fusion protein was detected by Western analysis.

分类号： Q5

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