Stress responses in the honeybee (Apis mellifera L.) midgut triggered by PFOA and PFHxS at environmental residue concentrations detected in bee products

文献类型： 外文期刊

第一作者： Cang, Tao

作者： Cang, Tao;Lv, Lu;Wang, Dou;Xu, Zhenlan;Wang, Xinquan;Wang, Yanhua;Yin, Yue;Shao, Kan

作者机构：

关键词： Pollination services; Emerging contaminants; Intestinal toxicity; Toxic mechanism

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：11.3； 五年影响因子：12.4 ）

ISSN： 0304-3894

年卷期： 2025 年 497 卷

页码：

收录情况： SCI

摘要： Perfluorooctanoic acid (PFOA) and perfluorohexane sulfonate (PFHxS), as persistent global contaminants, pose substantial threats to pollinators like honeybees (Apis mellifera L.), yet their sub-lethal effects remain insufficiently explored. Here, we exposed bees to environmentally relevant concentrations (0.5-50 mu g/L) of PFOA and PFHxS for 10 days, assessing midgut toxicity through pathology, metabolomics, and gene expression, and examined curcumin (Cur) as a potential mitigator. Although acute toxicity assays indicated relatively high LC50 values (266-331 mg/L), sub-lethal exposures induced pronounced midgut injury, characterized by decreased epithelial cell density and thinning of the protective gel layer. Disruption of glycerophospholipid metabolism was detected, impairing membrane integrity. Additionally, PFOA and PFHxS downregulated barrier integrity genes (ZO-2 and Mucin-1), while upregulating inflammatory (Eiger and Relish) and apoptotic indicators (Caspase-3, Caspase-8, and Caspase-9). For several sub-lethal endpoints, PFHxS triggered more pronounced effects than PFOA. Notably, curcumin treatment restored midgut integrity and reversed many of the associated molecular disruptions. These results demonstrated that environmentally relevant concentrations of PFAS could substantially compromise honeybee gut health, offering critical insights for ecological risk assessments aimed at safeguarding these essential pollinators.

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