Lactococcus intestinalis sp. nov., a new lactic acid bacterium isolated from intestinal contents in Alzheimer's disease mice

文献类型： 外文期刊

第一作者： Sun, Peilin

作者： Sun, Peilin;Li, Xue;Shi, Weixiong;Zhang, Ling;Su, Lei;Qin, Chuan;Sun, Peilin;Li, Xue;Shi, Weixiong;Zhang, Ling;Su, Lei;Qin, Chuan;Li, Ming

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关键词： Genomic characterisations; Polyphasic taxonomy; Lactococcus intestinalis sp; nov; Alzheimer's disease mice

期刊名称：ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY （ 影响因子：2.6； 五年影响因子：2.8 ）

ISSN： 0003-6072

年卷期： 2023 年 116 卷 5 期

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收录情况： SCI

摘要： A gram-positive, facultatively anaerobic, and coccoid or ovoid-shaped bacterium designated M2458(T) was isolated from the intestinal contents of APP(swe)/PS Delta E9 mouse model of Alzheimer's disease. With the polyphasic approach, the taxonomic position of the novel isolate was confirmed. Strain M2458(T) grew well at 37 degrees C on YCFA agar. Strain M2458(T) belongs to the family Streptococcaceae and class Bacilli, and it is closed to Lactococcus formosensis NBRC 109475(T) (97.59% sequence similarity) according to its 16S rRNA gene sequence. In a comparison of two housekeeping genes, rpoA and rpoB, strain M2458(T) was found to be well separated from Lactococcus formosensis NBRC 109475(T). On the basis of whole genome sequences, the DNA G+C content was 38.29 mol%. The phylogentic analysis of the whole genome showed that a different branch was clearly formed in the phylogenetic tree of strain M2458(T) compared to other strains in the genus Lactococcus. A total of eight genes in strain M2458(T) are involved in the 'neurodegenerative disease' pathway, which involves an annotated protein (glyceraldehyde 3-phosphate dehydrogenase) involved in Alzheimer's disease. In terms of average nucleotide identity and digital DNA-DNA hybridization, strain M2458(T) was identified as a novel species of the genus Lacococcus. The major fatty acids (> 10% of the total fatty acids) were C18:1 omega 9c (39.68%), C16:0 (13.26%) and C18:1 omega 7c (11.52%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and an unidentified phospholipid. As a result of its genotypic and phenotypic characteristics, strain M2458(T) was considered to be a new species within the genus Lactococcus; the name Lactococcus intestinalis sp. nov. has been proposed, with type strain M2458(T) (=JCM 35706 = CGMCC 1.60066).

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