An overview of single-molecule techniques and applications in the study of nucleic acid structure and function

文献类型： 外文期刊

第一作者： Fang, Junkang

作者： Fang, Junkang;Xie, Congbao;Tao, Yanfei;Wei, Dengguo;Fang, Junkang;Xie, Congbao;Tao, Yanfei;Wei, Dengguo;Fang, Junkang;Xie, Congbao;Tao, Yanfei;Wei, Dengguo;Fang, Junkang;Xie, Congbao;Tao, Yanfei;Wei, Dengguo;Fang, Junkang;Xie, Congbao;Tao, Yanfei;Wei, Dengguo;Fang, Junkang;Xie, Congbao;Tao, Yanfei;Wei, Dengguo;Tao, Yanfei;Wei, Dengguo

作者机构：

关键词： Single-molecule technology; Nucleic acid structure; Conformational distribution; Structural transformation; Interaction between proteins and nucleic; acid structures; Interaction between small molecules and; nucleic acid structures

期刊名称：BIOCHIMIE （ 影响因子：3.9； 五年影响因子：3.9 ）

ISSN： 0300-9084

年卷期： 2023 年 206 卷

页码：

收录情况： SCI

摘要： Nucleic acids are an indispensable component in all known life forms. The biological processes are regulated by Nucleic acids, which associate to form special high-order structures. since the high-level structures of nucleic acids are related to gene expression in cancer cells or viruses, it is very likely to become a potential drug target. Traditional biochemical methods are limited to distinguish the confor-mational distribution and dynamic transition process of single nucleic acid structure. The ligands based on the intermediate and transition states between different conformations are not designed by tradi-tional biochemical methods. The single-molecule techniques enable real-time observation of the indi-vidual nucleic acid behavior due to its high resolution. Here, we introduce the application of single-molecule techniques in the study of small molecules to recognize nucleic acid structures, such as single-molecule FRET, magnetic tweezers, optical tweezers and atomic force microscopy. At the same time, we also introduce the specific advantages of single-molecule technology compared with traditional biochemical methods and some problems arisen in current research.(c) 2022 Published by Elsevier B.V.

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