Anti-inflammatory activity of arctigenin against PCV2 infection in a mouse model

文献类型： 外文期刊

第一作者： Wu, Lijun

作者： Wu, Lijun;Chen, Jie;Chen, Xiabing;Shao, Zhiyong;Yang, Wenhai;He, Bin;Zhou, Danna;Chen, Runshan

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关键词： arctigenin; mice; NF-kappa B; PCV2; proinflammatory cytokine

期刊名称：VETERINARY MEDICINE AND SCIENCE （ 影响因子：1.772； 五年影响因子：1.885 ）

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年卷期： 2022 年 8 卷 2 期

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收录情况： SCI

摘要： Arctigenin (ACT) is a novel anti-inflammatory lignan extracted from Arctium lappa L, a herb commonly used in traditional Chinese herbal medicine. In this study, we investigated the molecular mechanism whereby ACT inhibits PCV2 infection-induced proinflammatory cytokine production in vitro and in vivo. We observed that in PCV2 infection+ACT treated PK-15 cells, proinflammatory cytokine production was significantly reduced, compared to the PCV2-infected cells. The transfection and luciferase reporter assay confirmed that ACT suppressed NF-kappa B signalling pathway activation following PCV2 infection in PK-15 cells. Furthermore, western blotting demonstrated that ACT suppressed the NF-kappa B signal pathway in PCV2 infection-stimulated PK-15 cells by inhibiting the translocation of p65 from the cytoplasm to the nucleus and I kappa B alpha phosphorylation. BALB/c mice were used as a model to evaluate the anti-inflammatory effect of ACT in vivo. We found that the BALB/c mice inoculated with PCV2 infection + ACT treated showed a significant reduction of proinflammatory cytokine production in serum, lung and spleen tissue, compared to the PCV2-infected mice. Western blotting confirmed that ACT suppressed the NF-kappa B signal pathway in PCV2-infected mice by inhibiting the translocation of p65 from the cytoplasm to the nucleus and I kappa B alpha phosphorylation in lung tissue. Our studies first demonstrate that ACT inhibits PCV2 infection-induced proinflammatory cytokine production by suppressing the phosphorylation and nuclear translocation of NF-kappa B in vitro and in vivo. These results will help further develop ACT as a Traditional Chinese herbal medicine remedy in the treatment of porcine circovirus-associated diseases.

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