A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water

文献类型： 外文期刊

第一作者： Shao, Yong

作者： Shao, Yong;Tian, Run;Wang, Miao;Cao, Jing;Cao, Zhen;Jin, Fen;She, Yongxin;Shao, Yong;Tian, Run;Wang, Miao;Cao, Jing;Cao, Zhen;Jin, Fen;She, Yongxin;Duan, Jiaqi;Li, Guangyue;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Abd El-Aty, A. M.

作者机构：

关键词： glyphosate; aptamer; qPCR; SYBR Green I; sensor

期刊名称：SENSORS （ 影响因子：3.9； 五年影响因子：4.1 ）

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年卷期： 2023 年 23 卷 2 期

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收录情况： SCI

摘要： Glyphosate (GLYP) is a broad-spectrum, nonselective, organic phosphine postemergence herbicide registered for many food and nonfood fields. Herein, we developed a biosensor (Mbs@dsDNA) based on carboxylated modified magnetic beads incubated with NH2-polyA and then hybridized with polyT-glyphosate aptamer and complementary DNA. Afterwards, a quantitative detection method based on qPCR was established. When the glyphosate aptamer on Mbs@dsDNA specifically recognizes glyphosate, complementary DNA is released and then enters the qPCR signal amplification process. The linear range of the method was 0.6 mu mol/L-30 mmol/L and the detection limit was set at 0.6 mu mol/L. The recoveries in tap water ranged from 103.4 to 104.9% and the relative standard deviations (RSDs) were <1%. The aptamer proposed in this study has good potential for recognizing glyphosate. The detection method combined with qPCR might have good application prospects in detecting and supervising other pesticide residues.

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