Evaluation and comparison of immune responses induced by two Mpox mRNA vaccine candidates in mice

文献类型： 外文期刊

第一作者： Yang, Xidan

作者： Yang, Xidan;Hu, Congxia;Yang, Xuetao;Yang, Xiu;Yuan, Yuan;Lin, Jihui;Yang, Xidan;Yang, Xuetao;Yang, Xiu;Liu, Cong;Wang, Peng George;Lin, Jihui;Hu, Xing;Wang, Xingyun;Liu, Cong;Wang, Peng George;Du, Shouwen

作者机构：

关键词： antigen proteins; immune response; Mpox virus; mRNA vaccine; one-component and multicomponent

期刊名称：JOURNAL OF MEDICAL VIROLOGY （ 影响因子：12.7； 五年影响因子：8.5 ）

ISSN： 0146-6615

年卷期： 2023 年 95 卷 10 期

页码：

收录情况： SCI

摘要： The epidemic of Mpox virus (MPXV) from May 2022 was once declared as a Public Health Emergency of International Concern by the World Health Organization. Vaccines play an important role in prevention of infectious diseases, and mRNA vaccine technology was proved to be a safe and effective platform with successful application in defense of coronavirus disease 2019. In this study, based on A29L, M1R, A35R, and B6R of MPXV, we developed two MPXV mRNA vaccine candidates, designated as MPXfus and MPXmix. The MPXfus was one-component, in which these four antigen proteins were linked in tandem by flexible linker and encoded by an individual mRNA as a fusion protein. The MPXmix was multicomponent containing four mRNA, and each mRNA encoded one antigen protein respectively. Mice were immunized with equal quality of MPXfus or MPXmix, delivered by lipid nanoparticles for evaluation and comparison of the immune responses induced by these two MPXV vaccine candidates. Results of immune response analyses indicated that both MPXfus and MPXmix could elicit high-level of antigen-specific antibodies and robust cellular immune response in mice. Moreover, results of virus neutralization assays suggested that sera from MPXfus- or MPXmix-immunized mice possessed high neutralizing activities against vaccinia virus. In addition, titers of antigen-specific antibody, levels of cellular immune response, and activities of neutralizing antibody against vaccinia virus induced by MPXfus and MPXmix presented no significant difference. In summary, this study provides valuable insights for further clinical development of one-component and multicomponent mRNA vaccine candidates for the prevention of MPXV and other orthomyxoviruses.

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