A spatiotemporal transcriptomic network dynamically modulates stalk development in maize

文献类型： 外文期刊

第一作者： Le, Liang

作者： Le, Liang;Guo, Weijun;Du, Danyao;Zhang, Xiaoyuan;Wang, Weixuan;Yu, Jia;Wang, Huan;Zhang, Chunyi;Pu, Li;Le, Liang;Pu, Li;Qiao, Hong;Qiao, Hong;Zhang, Chunyi

作者机构：

关键词： transcriptome; stalk; plant height; maize; ZmD1

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.263； 五年影响因子：11.619 ）

ISSN： 1467-7644

年卷期： 2022 年 20 卷 12 期

页码：

收录情况： SCI

摘要： Maize (Zea mays) is an important cereal crop with suitable stalk formation which is beneficial for acquiring an ideal agronomic trait to resist lodging and higher planting density. The elongation pattern of stalks arises from the variable growth of individual internodes driven by cell division and cell expansion comprising the maize stalk. However, the spatiotemporal dynamics and regulatory network of the maize stalk development and differentiation process remain unclear. Here, we report spatiotemporally resolved transcriptomes using all internodes of the whole stalks from developing maize at the elongation and maturation stages. We identified four distinct groups corresponding to four developmental zones and nine specific clusters with diverse spatiotemporal expression patterns among individual internodes of the stalk. Through weighted gene coexpression network analysis, we constructed transcriptional regulatory networks at a fine spatiotemporal resolution and uncovered key modules and candidate genes involved in internode maintenance, elongation, and division that determine stalk length and thickness in maize. Further CRISPR/Cas9-mediated knockout validated the function of a cytochrome P450 gene, ZmD1, in the regulation of stalk length and thickness as predicted by the WGCN. Collectively, these results provide insights into the high genetic complexity of stalk development and the potentially valuable resources with ideal stalk lengths and widths for genetic improvements in maize.

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