文献类型： 外文期刊
第一作者： Zhao, Shanshan
作者： Zhao, Shanshan;Guan, Yudong;Zhao, Shanshan;Schlueter, Hartmut;Du, Shouwen;Du, Shouwen;Guan, Yudong;Guan, Yudong
作者机构：
关键词： N-glycomics; N-sialoglycans; nMatch; Mass spectrometry
期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：3.3； 五年影响因子：3.1 ）
ISSN： 0273-2289
年卷期： 2025 年
页码：
收录情况： SCI
摘要： The structural instability and electronegativity of sialic acids make N-sialoglycan profiling challenging. We compared commonly used preparation strategies, including native, acetohydrazide-based derivatization, and permethylation, in N-glycomics with nMatch, an R-based bioinformatics tool that ensures full coverage of the detected N-glycans using mass spectrometry. We demonstrated that permethylation leads to efficient detection of not only N-sialoglycans but also other N-glycans and, therefore, provides a valuable reference for the selection of the appropriate N-glycan preparation strategy in future studies.
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