Genome-wide association and RNA-seq analyses identify loci for pod orientation in rapeseed (Brassica napus)

文献类型： 外文期刊

第一作者： Yang, Yuting

作者： Yang, Yuting;Wang, Wenxiang;Hu, Qiong;Liu, Jia;Yang, Yuting;Wang, Wenxiang;Hu, Qiong;Liu, Jia;Raman, Harsh

作者机构：

关键词： rapeseed; pod orientation; GWAS; RNA-seq; candidate genes

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 13 卷

页码：

收录情况： SCI

摘要： Spatial distribution and orientation of pods on the main raceme (stem) and branches could affect rapeseed yield. However, genomic regions underlying the pod orientation were not described in Brassica species. Here, we determined the extent of genetic variation in pod orientation, described as the angles of pedicel on raceme (APR) and angles of the pod on pedicel (APP) among 136 rapeseed accessions grown across three environments of the upper, middle and lower Yangtze River in China. The APR ranged from 59 degrees to 109 degrees, while the APP varied from 142 degrees to 178 degrees. Statistical analysis showed that phenotypic variation was due to genotypic (G) and environmental (E) effects. Using the genome-wide association analysis (GWAS) approach, two QTLs for APR (qBnAPR.A02 and qBnAPR.C02) and two for APP (qBnAPP.A05 and qBnAPP.C05), having minor to moderate allelic effects (4.30% to 19.47%) were identified. RNA-seq analysis revealed 606 differentially expressed genes (DEGs) in two rapeseed accessions representing the extreme phenotypes for pod orientation and different alleles at the QTLs of APR. Three DEGs (BnLAZY4.A02, BnSAUR32.A02, and BnSAUR32.C02) were identified as the most likely candidates responsible for variation in pod orientation (APR). This study elucidates the genomic regions and putative candidate genes underlying pod orientation in B. napus.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序