Identification and characterization of a calcium-binding peptide from salmon bone for the targeted inhibition of α-amylase in digestion

文献类型： 外文期刊

第一作者： Xu, Zhe

作者： Xu, Zhe;Han, Shiying;Yan, Xu;Li, Tingting;Cui, Na;Xu, Zhe;Tan, Zhijian;Xu, Zhe;Wu, Jianping;Liu, Hanxiong;Du, Ming;Chen, Hongrui

作者机构： Dalian Minzu Univ, Coll Life Sci, Key Lab Biotechnol & Bioresources Utilizat, Minist Educ, Dalian 116600, Peoples R China;Liuzhou Inst Technol, Dept Food & Chem Engn, Liuzhou 545616, Guangxi, Peoples R China;Chinese Acad Agr Sci, Inst Bast Fiber Crops & Ctr Southern Econ Crops, Changsha 410205, Peoples R China;Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G2P5, Canada;Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Collaborat Innovat Ctr Seafood Deep Proc, Sch Food Sci & Technol, Dalian 116034, Peoples R China;Xihua Univ, Sch Food & Bioengn, Food Microbiol Key Lab Sichuan Prov, Chongqing Key Lab Special Food Co Built Sichuan &, Chengdu 611130, Sichuan, Peoples R China

关键词： Targeted inhibition; alpha-amylase; Calcium-binding; Salmon bone; Peptide

期刊名称：FOOD CHEMISTRY-X （ 2022影响因子：6.1； 五年影响因子：6.4 ）

ISSN： 2590-1575

年卷期： 2024 年 22 卷

页码：

收录情况： SCI

摘要： alpha-Amylase, essential for carbohydrate digestion, relies on calcium (Ca) for its structural integrity and enzymatic activity. This study explored the inhibitory effect of salmon bone peptides on alpha-amylase activity through their interaction with the enzyme's Ca -binding sites. Among the various salmon bone hydrolysates, salmon bone trypsin hydrolysate (SBTH) exhibited the highest alpha-amylase inhibition. The peptide IEELEEELEAER (PIE), with a sequence of Ile-Glu-Glu-Leu-Glu-Glu-Glu-Glu-Leu-Glu-Ala-Glu-Arg from SBTH, was found to specifically target the Ca -binding sites in alpha-amylase, interacting with key residues such as Asp206, Trp203, His201, etc. Additionally, cellular experiments using 3 T3 -L1 preadipocytes indicated PIE's capability to suppress adipocyte differentiation, and decreases in intracellular triglycerides, total cholesterol, and lipid accumulation. In vivo studies also showed a significant reduction in weight gain in the group treated with PIE(6.61%)compared with the control group (33.65%). These findings suggest PIE is an effective alpha-amylase inhibitor, showing promise for obesity treatment.

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