Actinoplanes flavus sp. nov., a novel cellulase- producing actinobacterium isolated from coconut palm rhizosphere soil

文献类型： 外文期刊

第一作者： Luo, Xianxian

作者： Luo, Xianxian;Sun, Xiujun;Huang, Zhenzhen;He, Chuan;Zhao, Junwei;Xiang, Wensheng;Song, Jia;Wang, Xiangjing;Xiang, Wensheng

作者机构：

关键词： Actinoplanes flavus sp. nov.; draft genome; polyphasic analysis; 16S rRNA gene

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.689； 五年影响因子：2.925 ）

ISSN： 1466-5026

年卷期： 2021 年 71 卷 9 期

页码：

收录情况： SCI

摘要： A novel cellulase-producing actinomycete, designated strain NEAU-H7(T), was isolated from coconut palm rhizosphere soil collected from Wenchang City, Hainan Province, PR China. A polyphasic taxonomic study was carried out to establish the status of this strain. Results of 16S rRNA gene sequence analysis indicated that strain NEAU-H7(T) belonged to the genus Actinoplanes, with highest similarity to Actinoplanes hulinensis NEAU-M9(T) (99.2 % 16S rRNA gene sequence similarity). The diagnostic sugars in cell hydrolysates were determined to be ribose, galactose and mannose. The major fatty acids (>10%) were C-16:0, C-18:1 omega 9c and C-18:0. The predominant menaquinones were identified as MK-9(H-4) and MK-9(H-6). The major polar lipids were phosphatidylethanolamine, phosphatidylinositol and two phosphatidylinositol mannosides. The amino acid of the cell-wall peptidoglycan was determined to be meso-diaminopimelic acid. The DNA G+C content was 71.2 mol%. Phylogenetic analysis using 16S rRNA gene sequences showed that strain NEAU-H7(T) formed a stable phyletic line with A. hulinensis NEAU-M9(T). However, wholegenome phylogeny showed strain NEAU-H7(T) formed a stable phyletic line with A. hulinensis NEAU-M9(T) (99.2%), Actinoplanes campanulatus DSM 43148(T) (98.6%), Actinoplanes capillaceus DSM 44859(T) (98.3%) and Actinoplanes lobatus DSM 43150(T) (97.6%). The digital DNA-DNA hybridization (dDDH) results between them were 53.6 (50.9-56.2), 54.1 (51.3-56.9), 53.1 (50.3-55.9) and 52.9 % (50.1-55.6 %), and wholegenome average nucleotide identity (ANI) values between them were 93.7, 93.6, 93.5 and 93.5 %. The low dDDH and ANI values demonstrated that strain NEAU-H7(T) could be distinguished from its reference strains. Moreover, genomic analysis indicated that the strain NEAU-H7(T) had the potential to decompose cellulose and produce bioactive compounds. On the basis of morphological, chemotaxonomic and phylogenetic characteristics, strain NEAU-H7(T) is proposed to represent a novel species of the genus Actinoplanes, with the name Actinoplanes flavus sp. nov. The type strain is NEAU-H7(T) (=CCTCC AA 2020034(T)=DSM 112042(T)).

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