Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate
文献类型： 外文期刊
第一作者： Tian, Yong-Sheng
作者： Tian, Yong-Sheng;Xiong, Ai-Sheng;Xu, Jing;Zhao, Wei;Gao, Feng;Fu, Xiao-Yan;Xu, Hu;Zheng, Jian-Li;Peng, Ri-He;Yao, Quan-Hong
作者机构：
期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.792； 五年影响因子：5.26 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： Applying the genomic library construction process and colony screening, a novel aroA gene encoding 5-enopyruvylshikimate-3-phosphate synthase from Ochrobactrum anthropi was identified, cloned, and overexpressed, and the enzyme was purified to homogeneity. Furthermore, site-directed mutagenesis was employed to assess the role of single amino acid residues in glyphosate resistance.
分类号： Q939.9
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