Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate
文献类型: 外文期刊
第一作者: Tian, Yong-Sheng
作者: Tian, Yong-Sheng;Xiong, Ai-Sheng;Xu, Jing;Zhao, Wei;Gao, Feng;Fu, Xiao-Yan;Xu, Hu;Zheng, Jian-Li;Peng, Ri-He;Yao, Quan-Hong
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期刊名称:APPLIED AND ENVIRONMENTAL MICROBIOLOGY ( 影响因子:4.792; 五年影响因子:5.26 )
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收录情况: SCI
摘要: Applying the genomic library construction process and colony screening, a novel aroA gene encoding 5-enopyruvylshikimate-3-phosphate synthase from Ochrobactrum anthropi was identified, cloned, and overexpressed, and the enzyme was purified to homogeneity. Furthermore, site-directed mutagenesis was employed to assess the role of single amino acid residues in glyphosate resistance.
分类号: Q939.9
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