Degradation of DDT by a Novel Bacterium, Arthrobacter globiformis DC-1: Efficacy, Mechanism and Comparative Advantage

文献类型: 外文期刊

第一作者: Wang, Xiaoxu

作者: Wang, Xiaoxu;Wang, Hui;Luo, Qing;Wu, Hao;Sun, Lina;Oba, Belay Tafa;Luo, Qing;Luo, Qing;Luo, Qing;Liu, Jiaxin;Tang, Lanxin;Yang, Miao

作者机构: Shenyang Univ, Key Lab Ecorestorat Reg Contaminated Environm, Minist Educ, Shenyang 110044, Peoples R China;Arba Minch Univ, Coll Nat & Computat Sci, Dept Chem, Arba Minch, Ethiopia;China Geol Survey, Northeast Geol S&T Innovat Ctr, Shenyang 110034, Peoples R China;Minist Nat Resources, Key Lab Black Soil Evolut & Ecol Effect, Shenyang 110034, Peoples R China;Liaoning Acad Agr Sci, Shenyang 110161, Peoples R China;China West Normal Univ, Sichuan Prov Engn Lab Monitoring & Control Soil Er, Nanchong 637009, Peoples R China

关键词: DDT; biodegradation; Arthrobacter globiformis; bioremediation

期刊名称:WATER ( 2022影响因子:3.4; 五年影响因子:3.5 )

ISSN:

年卷期: 2023 年 15 卷 15 期

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收录情况: SCI

摘要: A novel bacterium, Arthrobacter globiformis DC-1, capable of degrading DDT as its sole carbon and energy source, was isolated from DDT-contaminated agricultural soil. The bacterium can degrade up to 76.3% of the DDT at a concentration of 10 mg/L in the mineral salt medium (MSM) within 1 day of incubation. The effects of various environmental conditions, such as the concentration of DDT, temperature, pH and additional carbon sources, on its growth and biodegrading capacity of DDT were investigated in the MSM. The A. globiformis DC-1 strain could efficiently grow and degrade DDT at a wide range of concentrations, with the maximum growth and degradation rate at 10 mg/LDDT, followed by inhibitory effects at higher concentrations (20 and 30 mg/LDDT). Mesophilic temperatures (25-30 & DEG;C) and a pH of 7-7.5 were the most suitable conditions for the growth and biodegradation. The presence of carbon sources significantly increased the growth of the DC-1 strain; however, degradation was inhibited in the present of glucose, sucrose and fructose, and peptone was determined to be the most appropriate carbon source for A. globiformis DC-1. The optimal DDT degradation (84.2%) was observed at 10 mg/LDDT, peptone as carbon source in pH 7.5 at 30 & DEG;C with 1 day of incubation. This strain could also degrade DDE, DDD and DDT simultaneously as the sole carbon and energy source, with degradation rates reaching 70.61%, 64.43% and 60.24% in 10 days, respectively. The biodegradation pathway by A. globiformis DC-1 revealed that DDT was converted to DDD and DDE via dechlorination and dehydrochlorination, respectively; subsequently, both DDD and DDE transformed to DDMU through further dechlorination, and finally, after ring opening, DDMU was mineralized to carbon dioxide. No intermediate metabolites accumulation was observed during the GC/MS analysis, demonstrating that the A. globiformis DC-1 strain can be used for the bioremediation of DDT residues in the environment.

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