Nitrogen fixation genetics and regulation in a pseudomonas stutzeri strain associated with rice
文献类型: 外文期刊
第一作者: Desnoues, N
作者: Desnoues, N;Lin, M;Guo, XW;Ma, LY;Carreno-Lopez, R;Elmerich, C
作者机构:
期刊名称:MICROBIOLOGY-SGM ( 影响因子:2.777; 五年影响因子:2.871 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: The Pseudomonas stutzeri strain A1501 (formerly known as Alcaligenes faecalis) fixes nitrogen under microaerobic conditions in the free-living state and colonizes rice endophytically. The authors characterized a region in strain A1501, corresponding to most of the nif genes and the rnf genes, involved in electron transport to nitrogenase in Rhodobacter capsulatus. The region contained three groups of genes arranged in the same order as in Azotobacter vinelandii: (1) nifB fdx ORF3 nifQ ORF5 ORF6; (2) nifLA-mfABCDGEF-nifY2/nafY; (3) ORF13 ORF12-nifHDK-nifTY ORF1 ORF2-nifEN. Unlike in A. vinelandii, where these genes are not contiguous on the chromosome, but broken into two regions of the genome, the genes characterized here in P. stutzeri are contiguous and present on a 30 kb region in the genome of this organism. Insertion mutagenesis confirmed that most of the nif and the rnf genes in A1501 were essential for nitrogen fixation. Using lacZ fusions it was found that nif and rnf gene expression was under the control of ntrBC, nifLA and rpoN and that the rnf gene products were involved in the regulation of the nitrogen fixation process.
分类号: Q93
- 相关文献
作者其他论文 更多>>
-
Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China
作者:Sun, YC;Chen, YC;Tian, ZX;Li, FM;Wang, XY;Zhang, J;Xiao, ZL;Lin, M;Gilmartin, N;Dowling, DN;Wang, YP
关键词:
-
Diversity of degradation pathways of some aromatic compounds by phenotype and genotype testing in Acinetobacter strains
作者:Yu, XQ;Belhaj, A;Elmerich, C;Lin, M
关键词:Acinetobacter;aromatic compounds;degrading diversity;ERIC-PCR;16S-23S rRNA intergenic spacer sequences
-
A feasibility study on diagnosing wheat water status using spectral reflectance
作者:Tian, QJ;Gong, P;Zhao, CJ;Guo, XW
关键词:wheat leaf;spectroradiometer;reflectance;absorption feature;relative water content;water status
-
ATTACHMENT OF ALCALIGENES TO RICE ROOTS
作者:YOU, CB;LIN, M;FANG, XJ;SONG, W
关键词:
-
Construction and characterization of partially ntrC-deleted mutants in Alcaligenes faecalis
作者:Cheng, HM;Lin, M;Ping, SZ;Jia, SR
关键词:Alcaligenes faecalis;ntrC mutant;expression and regulation of nif genes
-
Particle flux through the Huanghai Sea cold water mass
作者:Guo, XW;Zhang, YS
关键词:settling particulate matter;particle flux model;resuspension ratio;thermocline;Huanghai aea cold water mass
-
Functional identification of gene cluster for the aniline metabolic pathway mediated by transposable element
作者:Liang, QF;Chen, M;Xu, YQ;Zhang, W;Ping, SZ;Lu, W;Song, XL;Wang, WW;Geng, LZ;Masahiro, T;Lin, M
关键词:aniline;biodegradation;transposable element;gene cluster