High uptake of citrus essential oil-loaded zein particles into E. coli tuned by the wet-crosslinked folic acid

文献类型： 外文期刊

第一作者： Yang, Feilong

作者： Yang, Feilong;Zhao, Chengying;Feng, Liping;Wang, Yanqi;Ma, Mengyu;Zheng, Jinkai;Yang, Feilong;Fogliano, Vincenzo;Madadlou, Ashkan

作者机构：

关键词： Biopolymer; Covalent bond; Protein modification; Plant protein; E; coli

期刊名称：FOOD HYDROCOLLOIDS （ 影响因子：10.7； 五年影响因子：10.9 ）

ISSN： 0268-005X

年卷期： 2023 年 142 卷

页码：

收录情况： SCI

摘要： Zein particles are increasingly used for encapsulation and delivery of hydrophobic bioactive. Conjugation of zein with multi-carboxylic acids by the alkali-catalysed and wet amidation process causes zein crosslinking and en-ables tuning the characteristics of zein particles. However, little is known about how the conjugation is asso-ciated with the crosslinking mode, i.e., intra and/or intermolecular crosslinks. In this study, folic acid (FoA) was conjugated with zein via the wet method, and citrus essential oil (CEO) was encapsulated within the constructed zein-FoA particles. Infra-red spectroscopy and nuclear magnetic resonance analysis indicated amide bond for-mation between zein and FoA showing that the content of primary amino groups in zein particles decreased due to conjugation. The mole fraction of FoA in the particles (XFoA) was measured as an index of conjugation extent and even though it did not exceed-0.1, conjugation influenced several characteristics of the particles. The contents of alpha-helix and beta-turn structures decreased, and that of beta-sheets increased with increasing XFoA. We hypothesized based on zeta-potential and hydrodynamic diameter results, as well as gel electrophoresis that conjugation with FoA caused formation of only intramolecular crosslinks at XFoA <= 0.035, but it additionally resulted in intermolecular crosslinks at XFoA >= 0.037. Conjugation with FoA at XFoA 0.091 resulted in an improvement of the zein particles biological performance. It caused an increase (-30%) in the particle uptake by E. coli cells, as well as the magnitude of CEO delivery into the bacteria cells compared with non-conjugated particles.

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