Characterization of wheat oligo-tiller mutant ot2 and fine mapping of the mutant gene Taot2

文献类型： 外文期刊

第一作者： Wang, Chenxi

作者： Wang, Chenxi;Guo, Xinmei;Wang, Chenxi;Bai, Jiaxing;Xiong, Hongchun;Xie, Yongdun;Gu, Jiayu;Zhao, Linshu;Li, Huiyuan;Ding, Yuping;Guo, Huijun;Liu, Luxiang

作者机构：

期刊名称：PLANT GENOME （ 影响因子：3.8； 五年影响因子：4.2 ）

ISSN：

年卷期： 2025 年 18 卷 2 期

页码：

收录情况： SCI

摘要： Tiller number is a crucial determinant of grain yield in wheat (Triticum aestivum L.), and identifying functional alleles can enhance our understanding of wheat tiller development. Here, we describe the ot2 wheat mutant, which exhibits a 91% reduction in tiller number compared to the wild type and displays inhibited tiller bud differentiation from the 2-3 leaf stage. Bulked segregant analysis combined with exon sequencing mapped the Taot2 gene to chromosome 1BL. Fourteen kompetitive allele-specific PCR markers were developed and utilized to narrow down the region containing Taot2 to a 2.22 Mb interval. This region encompasses 39 high-confidence genes. Through transcriptomic analysis and functional gene investigation, TraesCS1B03G1126300 was identified as the candidate gene, encoding an auxin-responsive protein of the auxin/indole-3-acetic acid family. Our findings provide the foundation for map-based cloning of Taot2, a novel wheat tiller gene, and offer valuable insights into wheat tiller bud initiation.

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