Portable detection of Salmonella in food of animal origin via Cas12a-RAA combined with an LFS/PGM dual-signaling readout biosensor

文献类型： 外文期刊

第一作者： Wang, Yuanshang

作者： Wang, Yuanshang;Cao, Jianfang;Du, Pengfei;Wang, Weiting;Hu, Peng;Liu, Yaobo;Ma, Yanli;Wang, Yuanshang;Wang, Xiaohong;Wang, Yuanshang;Wang, Xiaohong;Abd El-Aty, A. M.;Abd El-Aty, A. M.

作者机构：

关键词： CRISPR/Cas12a; Food of animal origin; Salmonella; Lateral flow strips (LFS); Personal glucometer (PGM)

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.3； 五年影响因子：4.9 ）

ISSN： 0026-3672

年卷期： 2024 年 191 卷 10 期

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收录情况： SCI

摘要： A highly specific and sensitive rapid two-signal assay was developed for the detection of Salmonella typhimurium in foods of animal origin. The invA gene of Salmonella was used as the biorecognition element and recombinase-assisted amplification (RAA) technology for signal amplification. By utilizing the specific recognition and efficient trans-cleavage activity of CRISPR/Cas12a, point-of-care testing (POCT) for S. typhimurium was achieved via lateral flow strips (LFS) and personal glucometer (PGM) biosensors as dual signal readout systems, with sensitivities of 33 CFU/mL and 20 CFU/mL, respectively. Users can select the appropriate test system on the basis of specific application requirements: LFSs are ideal for rapid onsite screening, whereas glucometer biosensors offer precise quantitative determination. This approach simplifies the use of large instruments and overcomes site constraints, demonstrating good accuracy and applicability in animal-derived samples, with significant potential for the detection of other pathogens and for use in restricted environments.

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