Evaluation of a primer-probe energy transfer real-time PCR assay for detection of classical swine fever virus

文献类型： 外文期刊

第一作者： Zhang, Xing-Juan

作者： Zhang, Xing-Juan;Qiu, Hua-Ji;Zhang, Xing-Juan;Xia, Hongyan;Belak, Sandor;Xia, Hongyan;Belak, Sandor;Widen, Frederik;Liu, Lihong;Everett, Helen;Sosan, Olubukola;Crooke, Helen

作者机构：

关键词： RNA

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

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年卷期：

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收录情况： SCI

摘要： This study describes evaluation of a real-time PCR assay based on primer-probe energy transfer (PriProET) technology for detection of classical swine fever virus (CSFV). The PriProET technology allows melting curve analysis following PCR amplification and thus provides a higher specificity. The assay was compared with a TaqMan assay by testing a total of 203 samples including 175 clinical specimens and 28 batches of Hog Cholera Lapinized Virus (HCLV) vaccine. The two assays gave the same results for 184 (91%) samples. Compared with the TaqMan assay, 19 additional samples were found to be positive for CSFV using the PriProET assay. In an RNA mixture of both wild type CSFV and C-strain vaccine, the melting curves displayed only one curve: either a wild type-like or a vaccine-like depending on the dominating RNA. The PriProET assay can be a routine molecular tool or a confirmative tool for diagnosis of classical swine fever (CSF), especially in the case of samples that yield an inconclusive result by the TaqMan assay

分类号： R37

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