Identification of retrotransposon families and analysis of their transcriptional activation in wheat

文献类型： 外文期刊

第一作者： Tang, YM

作者： Tang, YM;Ma, YZ;Li, LC;Xin, ZY

作者机构：

关键词： Triticeae;wheat;retrotranspons;reversetranscriptase domain;evolution

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

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收录情况： SCI

摘要： Variations in the reverse transcriptase (RT) domain were analyzed to clarify retrotransposon family structure and their evolution in wheat genome. RT-PCR was conducted by using primers based on the conserved RT pep-tide motifs of plant retrotransposons to amplify the RT domain of retrotransposons in the seedlings of wheat line Pm97034 treated with powdery mildew fungus. High level of heterogeneity was detected in 51 (RT1-51) clones randomly selected and the identity of nucleotide sequence among them ranged from 75.4 percent to 97.9 percent. These sequences, in combination with previously identified seven representatives from wheat retrotransposon families (families 1-7), were used to construct a composite phylogenetic tree. Three new families, designated family 8 to family 10, were identified. Famliy 8 formed before divergence of the Pooideae subfamily and was regarged as an ancient retrotransposon family. Some members of family 4 and family 7 had transcriptional activativity, and were present with multiple copies in wheat and its related species.

分类号： N1

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