Comparison on Characterization of Longan (Dimocarpus longan Lour.) Polyphenoloxidase Using Endogenous and Exogenous Substrates

文献类型： 外文期刊

第一作者： Zhang, Ezhen

作者： Zhang, Ezhen;Li, Zhichun;Wang, Zhenxing;Li, Changbao;Sun, Jian;Xu, Liangxiong

作者机构：

关键词： Longan (Dimocarpus longan Lour.);polyphenoloxidase;endogenous substrate;exogenous substrate;characterization

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

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收录情况： SCI

摘要： Longan polyphenoloxidase (PPO) was extracted and partially purified using ammonium sulfate precipitation and dialysis. The PPO characterizations were compared using endogenous substrate (-)-epicatechin and exogenous substrate catechol. The optimal pH and optimal temperature for the PPO activity were different when reacting with both substrates. The addition qf ethylenediaminete-traacetic acid disodium salt into both substrate-enzyme systems exhibited the same lowest inhibition of the PPO activity. L-Ascorbic acid and L-cysteine were the best inhibitors to endogenous substrate-enzyme system, while L-ascorbic acid and glutathione were most effective inhibitors to exogenous substrate-enzyme system. Cupric (Cu~(2+)), ferric (Fe~(3+)), and ferrous (Fe~(2+)) ions accelerated the enzymatic-catalyzed reactions of both substrates. Kinetic analysis indicated that longan PPO strongly bound endogenous substrate but possessed a higher catalytic efficiency to exogenous substrate, and moreover, (-)-epicatechin was determined as the optimal substrate for longan PPO. This study is useful to exactly illuminate the enzymatic-catalyzed browning mechanism of postharvest longan fruit.

分类号： R15

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