C-13 NMR detection of non-protein nitrogen substance adulteration in animal feed

文献类型： 外文期刊

第一作者： Zhao, Chengxiang

作者： Zhao, Chengxiang;Sun, Yongyue;Zhao, Chengxiang;Wang, Tongtong;Chen, Furong;Chen, Gang

作者机构：

关键词： Animal feed; Adulteration; Non-protein nitrogen; C-13 NMR

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.478； 五年影响因子：4.29 ）

ISSN： 1618-2642

年卷期： 2022 年 414 卷 7 期

页码：

收录情况： SCI

摘要： Illegal adulteration of melamine in animal feed and food has been widely studied. However, the risk of using substitute non-protein nitrogen substances still exists. In this study, we developed the C-13 NMR method for the detection of non-protein nitrogen substance adulteration in animal feed. Three compounds, i.e., urea, melamine, and biuret, were used for method development. We found that the chemical shifts of the characteristic peaks in the carbon spectra of high-nitrogen adulterants were all between 150 and 170 ppm, whereas the chemical shifts of real protein peptide bonds (-CO-NH-) were between 170 and 180 ppm, demonstrating a good distinction between non-protein nitrogen and authentic protein. The method for analyzing melamine, urea, and biuret was validated. The R-2 values were all above 0.99 within the calibration range of 0.05-2% (w/w). The limits of quantification of urea, melamine, and biuret were 0.0120%, 0.0660%, and 0.0806%, respectively. This method involves simple sample pretreatment and rapid detection while also providing high accuracy. All the sample information obtained by NMR detection does not require strict impurity removal. Compared with a previously reported H-1 NMR method, the developed C-13 NMR method does not require strict moisture removal to avoid active hydrogen exchange, and the interfering peak overlap is mitigated.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序