Using the Intrinsic Fluorescence of DNA to Characterize Aptamer Binding

文献类型： 外文期刊

第一作者： Lu, Chang

作者： Lu, Chang;Zheng, Jinkai;Lu, Chang;Lopez, Anand;Liu, Juewen

作者机构：

关键词： intrinsic fluorescence; DNA; aptamer; binding

期刊名称：MOLECULES （ 影响因子：4.927； 五年影响因子：5.11 ）

ISSN：

年卷期： 2022 年 27 卷 22 期

页码：

收录情况： SCI

摘要： The reliable, readily accessible and label-free measurement of aptamer binding remains a challenge in the field. Recent reports have shown large changes in the intrinsic fluorescence of DNA upon the formation of G-quadruplex and i-motif structures. In this work, we examined whether DNA intrinsic fluorescence can be used for studying aptamer binding. First, DNA hybridization resulted in a drop in the fluorescence, which was observed for A30/T30 and a 24-mer random DNA sequence. Next, a series of DNA aptamers were studied. Cortisol and Hg2+ induced fluorescence increases for their respective aptamers. For the cortisol aptamer, the length of the terminal stem needs to be short to produce a fluorescence change. However, caffeine and adenosine failed to produce a fluorescence change, regardless of the stem length. Overall, using the intrinsic fluorescence of DNA may be a reliable and accessible method to study a limited number of aptamers that can produce fluorescence changes.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序