Overexpression of MxFRO6, a FRO gene from Malus xiaojinensis, increases iron and salt tolerance in Arabidopsis thaliana

文献类型： 外文期刊

第一作者： Li, Yingmei

作者： Li, Yingmei;Zhong, Jiliang;Huang, Pengfei;Shao, Bangtao;Li, Wenhui;Xie, Liping;Han, Meina;Han, Deguo;Liu, Wanda;Wang, Yu

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关键词： Malus xiaojinensis; MxFRO6; Iron stress; Salt stress

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT （ 影响因子：2.347； 五年影响因子：2.404 ）

ISSN： 1054-5476

年卷期： 2022 年 58 卷 2 期

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收录情况： SCI

摘要： Iron (Fe) is one of the essential micronutrients required by all plants. In this study, a FRO gene was cloned from the iron-efficient genotype Malus xiaojinensis and named as MxFRO6. The MxFRO6 gene was 2238 bp in length, and the MxFRO6 protein contained 745 amino acids with a theoretical isoelectric point of 6.79, a predicted protein with a theoretical molecular mass of 82.71 kDa, and an overall average hydrophilicity coefficient of 0.413. Subcellular localization results showed that MxFRO6 protein was localized to the cell membrane. The expression level of MxFRO6 was higher in the new leaves and roots, which was markedly affected by salt treat, low-iron treat, and high-iron treat. When MxFRO6 was introduced into Arabidopsis thaliana, it greatly increased the iron and salt tolerance in transgenic plant. Increased expression of MxFRO6 in transgenic A. thaliana also resulted in higher activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), and higher contents of proline and chlorophyll, while malondialdehyde (MDA) content was lower, especially in response to iron and salt stress. We argued that MxFRO6 is a new member of the FRO genes, and it may function as a regulator in response to iron stress and salt stress in plants.

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