Highly efficient enzymatic enrichment of n-3 polyunsaturated fatty acid glycerides via interfacial biocatalysis in Pickering emulsions

文献类型： 外文期刊

第一作者： Wu, Liumei

作者： Wu, Liumei;Zhang, Yufei;Zheng, Mingming;Wu, Liumei;Li, Dongming;Bi, Fuyang;Zhang, Yufei;Zheng, Mingming;Yu, Chao

作者机构：

关键词： N-3 PUFAs enrichment; Immobilized enzyme; Pickering emulsion; Interfacial biocatalysis; Mesoporous silicon spheres

期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）

ISSN： 0308-8146

年卷期： 2025 年 470 卷

页码：

收录情况： SCI

摘要： A novel Pickering interfacial biocatalysis (PIB) system has been, for the first time, successfully applied for the enzymatic selective hydrolysis of algae oils and fish oils to enrich n-3 PUFAs glycerides. Lipase AY 400SD was identified and adsorbed on hydrophobic hollow core-shell silica nanoparticles, resulting in the formation of the immobilized enzyme AY 400SD@HMSS-C18. The biocatalyst was employed as an emulsifier to stabilize the water-in-oil Pickering emulsion, resulting in the successful construction of the PIB system. The newly developed PIB system resulted in a notable enhancement of the n-3 PUFAs content of the six oils, with a mean increase ranging from 9.17 % to 23.09 %, and with the recovery rate of n-3 PUFAs exceeded 90 %. The platform demonstrated to be stable and recyclable. The present research illustrated that the PIB has the potential to be a viable alternative for the purpose of enhancing the content of n-3 PUFAs in glycerides.

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