Hydrophobic-flexible rational modification strategy provides fructooligosaccharides activity from Aspergillus niger

文献类型： 外文期刊

第一作者： Chen, Gan-Lin

作者： Chen, Gan-Lin;Yang, Li-Fang;Chen, Gan-Lin;Chen, Jing;Chen, Gan-Lin;Chen, Jing;Zhao, Ling-Zhi;Lin, Bo;Zheng, Feng-Jin;Verma, Krishan K.

作者机构：

关键词： Fructosyltransferase; Fructooligosaccharide; Molecular docking; Structural analysis; Aspergillus niger

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.6； 五年影响因子：6.9 ）

ISSN： 0023-6438

年卷期： 2025 年 230 卷

页码：

收录情况： SCI

摘要： Fructooligosaccharides (FOS), linear fructose oligomers with health benefits, are synthesized through sucrose transfructosylation using fructosyltransferases (FTases). This study employed the computer-aided design to modify the hydrophobic-flexible active domain of Aspergillus niger FTases. Mutant 142P exhibited 22.56 % (45.55 mu kat) higher than wild-type FTases, with optimal conditions, i.e, 55 degrees C, pH 5.0, and CO2+. Kinetic analysis reduced Km value (6.174 +/- 0.15 M), attributed to weakened active domain-sucrose interactions. FOS conversion by 142P enhanced up to 0.17-29.20 %, yielding 14.92 % 1-Kestose, and 14.27 % Nystose. Molecular docking enhanced active domain flexibility (structural shift from green to red) and reduced glucose affinity as key factors boosting activity and FOS conversion. Hydrophobic-flexible modification proves effective for optimizing FTases in industrial applications. This strategy provides a scalable solution for tailoring Aspergillus niger enzymes to meet the growing demands of the prebiotic and functional food industries.

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