Entropy-driven self-assembled enzyme-DNA nanomatrix cascade DNAzyme-CRISPR/cas system for multiplexed enhancement of self-powered sensing platform for protein detection

文献类型： 外文期刊

第一作者： Ma, Yunzhi

作者： Ma, Yunzhi;Wu, Ye-Yu;Yan, Jun;Huang, Ke-Jing;Tan, Xue-Cai;Duan, Yun;Ya, Yu

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关键词： Entropy-driven self-assembly enzyme; CRISPR/Cas system; C-MoS 2 hollow nanorods

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2024 年 282 卷

页码：

收录情况： SCI

摘要： Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated Proteins (CRISPR/Cas) system can accurately identify and cleave target DNA sequences, while the effective combination of DNA nanomatrix and entropy-driven self-assembled enzymes can significantly enhance the sensitivity, stability, and diversified functionality of sensors through highly ordered molecular arrangement and spontaneous efficient assembly processes. Herein, a carbon-encapsulated MoS2 hollow nanorod (C-MoS2) with excellent conductivity and multiple active sites is used to construct bioanode of biofuel cell by integrating it with an entropy-driven selfassembled enzyme-DNA nanomatrix cascade DNAzyme-CRISPR/Cas system. When thrombin binds aptamer, it exposes the trigger strand on the anode, initiating chain displacement. This activates DNAzyme, triggering a cascade reaction that cleaves and releases the probe strand. The probe then binds CrRNA, forming a multimeric complex with Cas protein.When non-specific cleavage of the single strand occurs, it leads to the release of glucose oxidase (GOD) from the DNA matrix and causes a significant decrease in the value of the system's open-circuit voltage (EOCV). The EOCV values show a good negative correlation with the concentration of thrombin (TB) in the range of 0.00001-100 nM, achieving a limit of detection of 3.55 fM (S/N = 3).

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