Assessment on alien herbicide-resistant gene flow among crucifers by sexual compatibility

文献类型： 外文期刊

第一作者： Zhao, XX

作者： Zhao, XX;Lu, WP;Qi, CK;Pu, HM;Xia, QX;Lu, DL;Liu, GS;Wang, YP

作者机构：

关键词： herbicide-resistant GM rapeseed;cruciferous plants;aniline blue fluorescence;mentor method;sexual compatibility;gene flow

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

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收录情况： SCI

摘要： Cross-pollination high cross-compatibility are frequently found in many cruciferous plants, which implies that there might be potential risk of alien gene flow among crucifers. In this work, the alien glyphosate-resistant gene flow between GM (Genetically modified) rapeseed variety Q3 (Brassica napus) and 6 varieties from 5 cruciferous species was assessed by sexual compatibility through aniline blue fluorescence observation, manual cross, mentor pollination with the mixture pollen and DNA identification. The results indicated that the compatibility indices of two B. napus varieties, B. jounce var. multiceps Tsen et Lee and Chinese cabbage (B. chinensis) (as female) with Q3 (as male) were 16.15,12.77, 2.345 and 0.85, respectively, F_1 plants were by PCE and all of these samples were confirmed as positive. Crossing between Orychophragmus vialaceus, Des-curainia sophia (as female) and Q3 (as male) expressed high cross-incompatibility. Fluorescence microscope observation indicated that growing of pollen tube terminated at the upper 1/3 part of the style of O. violaceus on the of D. sophia. The mentor plants were further analyzed by PCR and all were shown to be negative. Under the neighboring growth and natural pollination conditions, the of gene flow to two B. napus varieties were 2.3278 percent and 2.1487 percent, respectively, B. juncea var. multiceps Tsen et Lee and Chinese cabbage were 1.0157 percent and 0.9236 percent, respectively. No flow was observed in O. violaceus and D. sophia. Frequency of gene flow was highly correlated with sexual compatibility. Due to possible alien gene flow among crucifers, special consideration care should be taken to grow GM rapeseed.

分类号： N1

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