Expression and characterization of recombinant gallinacin-9 and gallinacin-8 in Escherichia coli

文献类型： 外文期刊

第一作者： Ma, De-ying

作者： Ma, De-ying;Shan, An-shan;Liu, Sheng-wang;Han, Zong-xi;Li, Yi-jin

作者机构：

关键词： gallinacins;antimicrobial peptides;chick;fusion protein;antibacterial activity;EPITHELIAL BETA-DEFENSIN;ANTIMICROBIAL PEPTIDES;AVIAN HETEROPHILS;CHICKEN;FUSION;PROTEINS;BACTERIA;IDENTIFICATION;PURIFICATION;INFLAMMATION

期刊名称：PROTEIN EXPRESSION AND PURIFICATION （ 影响因子：1.65； 五年影响因子：1.548 ）

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收录情况： SCI

摘要： Gallinacins (Gal) are antimicrobial peptides that play significant roles in innate immunity in chickens. Two Gal genes-Gal-8 and Gal-9-were cloned and sequenced from chicken liver and tongue, respectively, by reverse transcriptase-polymerase chain reaction (RT-PCR). In addition, the mRNA expression of these genes has been demonstrated across a panel of chicken tissues. It was demonstrated that Gal-9 mRNA was highly expressed in the tongue and small intestine and moderately expressed in the chicken proventriculus, lung. liver, heart, spleen, and thymus. However, Gal-8 mRNA was highly expressed in the chick small intestine and liver, and moderately expressed in the chick tongue, and lung. The recombinant fusion proteins containing Gal-9 or Gal-9 and Gal-8, namely rGal-9 and rGal-9-Gal-8, were produced and purified, respectively. Both rGal-9 and rGal-9-Gal-8 were expressed as insoluble bodies and exhibited the expected antimicrobial activity against Escherichia coli and pathogenic Streptococci suis CAB strain, as determined by the measurement of the inhibition zone and a liquid growth inhibition assay. (C) 2007 Elsevier Inc. All rights reserved.

分类号： Q51

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