Fusion expression of bovine lactoferricin in Escherichia coli

文献类型： 外文期刊

第一作者： Feng, Xing-jun

作者： Feng, Xing-jun;Wang, Jian-hua;Shan, An-shan;Teng, Da;Yang, Ya-lin;Yao, Yi;Yang, Guan-pin;Shao, Yan-chun;Liu, Shuo;Zhang, Fan

作者机构：

关键词： bovine lactoferricin;antibacterial activity;factor Xa;thrombin;fusion expression;affinity chromatography;ANTIBACTERIAL PEPTIDES;ANTIMICROBIAL PEPTIDES;PROTEIN;GENE;MEMBRANE;DOMAIN;IDENTIFICATION;BUNGAROTOXIN;POLYPEPTIDES;PURIFICATION

期刊名称：PROTEIN EXPRESSION AND PURIFICATION （ 影响因子：1.65； 五年影响因子：1.548 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The drug resistance problem has been growing with the utilization of current antibiotics in feed and medical industries. LfcinB, a 25-amino acid antibacterial peptide derived from bovine lactoferrin, is one of potential alternatives of antibiotics. According to the bias of codon utilization of Escherichia coli, a fragment encoding LfcinB has been chemically synthesized, inserted into vector pGEX-4T-2 and expressed in E. coli. The antibacterial peptide was fused with GST with a protease cleavage site located between them. Two constructs with different cleavage sites were made. One construct, pGEX-Th-LfcinB, contains a thrombin cleavage site carried by the vector, and the other, pGEX-Th-Xa-LfcinB, contains a Factor Xa cleavage site which was introduced after the thrombin cleavage site. Fusion protein GST-Th-LfcinB protein was efficiently cleaved by thrombin, yielding recombinant LfcinB showing antibacterial activity. However, fusion protein GEX-Th-Xa-Lfcin B containing Factor Xa recognition site could not be cleaved by Factor Xa at the conditions tried in this study. Successful expression of LfcinB in E coli provides a possible method to produce LfcinB in large amounts. (c) 2005 Elsevier Inc. All rights reserved.

分类号： Q51

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