Localization, expression, and secretion pathway of diapause hormone in embryo and larva of the silkworm, Bombyx mori

文献类型： 外文期刊

第一作者： Sun, JS

作者： Sun, JS;Chen, FS;Xu, WH

作者机构：

关键词： diapause hormone;in situ hybridization;immunocytochemistry;embryo;larva;Bombyx mori

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this paper, the distribution and expression of diapause hormone (DH) at mRNA and protein levels in the central nervous system of Bombyx mori embryo and larva were studied using whole-mount in situ hybridization and immunocytochemistry. Whole-mount immunocytochemistry revealed that the distribution of the DH-like immunoreactivity was throughout the central nervous system including the brain, suboesophageal ganglion (SG) and thoracic ganglia (TG); and that the corpus cardiacum and terminal abdominal ganglion may be the site for DH release due to the presence of strong immunoreactivity. In situ hybridization with the probe labeled by digoxigenin shows that the Bom-DH mRNA was also localized in the mandibular, maxillary, labial cell clusters. In addition, a pair of lateral neurons in the SG and a pair of ventral midline neurons in each TG expressing the Bom-DH transcript were also identified. These results were consistent with the localization of Bom-DH mRNA in larva by in situ hybridization and the distribution of the gene by RT-PCR, which is some different from the results reported previously.

分类号： N1

  • 相关文献

[1]Changes of proteome and phosphoproteome trigger embryo-larva transition of honeybee worker (Apis mellifera ligustica). Gala, Alemayehu,Fang, Yu,Woltedji, Dereje,Zhang, Lan,Han, Bin,Feng, Mao,Li, Jianke.

[2]Niemann-Pick C2 Proteins: A New Function for an Old Family. Guo, Mengbuo,Liu, Yang,Pelosi, Paolo,Wang, Guirong,Ban, Liping,Song, Li-Mei. 2018

[3]Identification of a POU factor involved in regulating the neuron-specific expression of the gene encoding diapause hormone and pheromone biosynthesis-activating neuropeptide in Bombyx mori. Zhang, TY,Kang, L,Zhang, ZF,Xu, WH.

[4]Differentially expressed genes in white egg 2 mutant of silkworm, Bombyx mori, at early embryo development stages. Zhang, Guozheng,Lou, Chengfu,Zhang, Guozheng,Wei, Yadong,Xia, Dingguo,Zhang, Yeshun,Deng, Bo,Liu, Ziyu,Zhang, Jiekui,Zhang, Guozheng,Wei, Yadong,Xia, Dingguo,Zhang, Yeshun. 2011

[5]Molecular cloning, developmental expression, and tissue distribution of the gene encoding DH, PBAN and other FXPRL neuropeptides in Samia cynthia ricini. Wei, ZJ,Zhang, TY,Sun, JS,Xu, AY,Xu, WH,Denlinger, DL. 2004

[6]Protein-DNA interactions in the promoter region of the gene encoding diapause hormone and pheromone biosynthesis activating neuropeptide of the cotton bollworm, Helicoverpa armigera. Hong, B,Zhang, ZF,Tang, SM,Yi, YZ,Zhang, TY,Xu, WH.

[7]Immunolocalization of Odorant-Binding Proteins on Antennal Chemosensilla of the Peach Aphid Myzus persicae (Sulzer). Zhao, Li-Jing,Sun, Lei,Zhang, Shan-Gan,Ban, Li-Ping.

[8]General odorant-binding proteins and sex pheromone guide larvae of Plutella xylostella to better food. Liu, Yang,Pelosi, Paolo,Wang, Guirong,Ban, Liping,Song, Li-Mei.

[9]Molecular identification and differential expression of sensory neuron membrane proteins in the antennae of the black cutworm moth Agrotis ipsilon. Yang, Ruo-Nan,Guo, Meng-Bo,Wang, Gui-Rong,Wu, Kong-Ming,Guo, Yu-Yuan,Zhang, Yong-Jun,Zhou, Jing-Jiang.

[10]The antenna-specific odorant-binding protein AlinOBP13 of the alfalfa plant bug Adelphocoris lineolatus is expressed specifically in basiconic sensilla and has high binding affinity to terpenoids. Liu, Z-W.,Sun, L.,Xiao, H-J.,Gu, S-H.,Guo, Y-Y.,Zhang, Y-J.,Xiao, H-J.,Zhou, J-J.. 2014

[11]FUNCTIONAL CHARACTERIZATION AND IMMUNOLOCALIZATION OF ODORANT BINDING PROTEIN 1 IN THE LUCERNE PLANT BUG, Adelphocoris lineolatus (GOEZE). Wang, Gui-Rong,Zhang, Xue-Ying,Guo, Yu-Yuan,Zhang, Yong-Jun,Wang, Wei-Xuan,Zhang, Ziding,Zhou, Jing-Jiang. 2011

[12]Subcellular localization of endogenous IAA during poplar leaf rhizogenesis revealed by in situ immunocytochemistry. Dong, Ningguang,Hao, Yanbin,Dong, Ningguang,Gao, Ying,Pei, Dong,Dong, Ningguang,Yin, Weilun.

[13]Functional characterization of a pheromone-binding protein from rice leaffolder Cnaphalocrocis medinalis in detecting pheromones and host plant volatiles. Sun, X.,Zhao, Z. -F.,Zeng, F. -F.,Wang, M. -Q.,Sun, X.,Zhang, A.,Lu, Z. -X..

[14]Uniqueness of the Gossypium mustelinum Genome Revealed by GISH and 45S rDNA FISH. Qiong Wu,Fang Liu,Shaohui Li,Guoli Song,Chunying Wang,Xiangdi Zhang,Yuhong Wang,David Stelly,Kunbo Wang. 2013

[15]Expression Analysis and Binding Assays in the Chemosensory Protein Gene Family Indicate Multiple Roles in Helicoverpa armigera. Zhao-Qun Li,Shuai Zhang,Jun-Yu Luo,Jing Zhu,Jin-Jie Cui,Shuang-Lin Dong.

[16]Molecular Characterization of a New Wheat-Thinopyrum intermedium Translocation Line with Resistance to Powdery Mildew and Stripe Rust. Zhan, Haixian,Li, Guangrong,Pan, Zhihui,Yang, Zujun,Zhan, Haixian,Zhang, Xiaojun,Hu, Jin,Li, Xin,Qiao, Linyi,Guo, Huijuan,Chang, Zhijian,Jia, Juqing,Chang, Zhijian. 2015

[17]Improved approaches of in situ hybridization and in situ enzyme histochemistry for gene expression analyses in plants. Liu, QF,Hao, ZR,Shen, DL. 1998

[18]Development and identification of wheat-Ag. pulcherrimum addition line and substitution line with BYDV resistance. Wu, DL,Xin, ZY,Chen, X,Xu, HJ,Ma, YZ,Zhang, ZY. 1999

[19]Molecular characterization of Pod1 during sex development in Chinese tongue sole (Cynoglossus semilaevis). Wang, Linna,Zhu, Ying,Xu, Wenteng,Shao, Changwei,Dong, Zhongdian,Li, Hailong,Cui, Zhongkai,Meng, Liang,Guo, Hua,Tian, Yongsheng,Chen, Songlin,Wang, Linna,Xu, Wenteng,Shao, Changwei,Tian, Yongsheng,Chen, Songlin. 2017

[20]Hybridization of Aegilops caudata with Triticum durum, Triticum aestivum and the detection of alien chromatins. Kong, XY,Zhou, RH,Dong, YS,Jia, JZ. 1999

作者其他论文 更多>>

微信小程序