Recovery of infectious rabbit hemorrhagic disease virus from rabbits after direct inoculation with in vitro-transcribed RNA

文献类型: 外文期刊

第一作者: Liu, GQ

作者: Liu, GQ;Zhang, YY;Ni, Z;Yun, T;Sheng, ZT;Liang, HL;Hua, JG;Li, SM;Du, QY;Chen, JP

作者机构:

关键词: PROTEIN;CALICIVIRUS;PARTICLES;SEQUENCES;GENE

期刊名称:JOURNAL OF VIROLOGY ( 影响因子:5.103; 五年影响因子:5.078 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: We report the first full-length infectious clone of strain JX/CHA/97 of rabbit hemorrhagic disease virus (RHDV). The transcripts from the full-length cDNA clones were infectious when they were directly injected into rabbits. The sequence of the virus recovered from the rabbits was identical to that of the injected RNA transcripts. The cDNA clone was engineered to contain one silent nucleotide change to create an EcoRV site (A to T at nucleotide 2908). The genetic marker was retained in the recovered progeny virus. The transfection of RNA transcripts into RK-13 cells resulted in the synthesis of viral antigens, indicating that the cDNA clones were replication competent. This stable infectious molecular clone should be an important tool for developing a better understanding of the molecular biology and pathogenesis of RHDV.

分类号: Q93

  • 相关文献

[1]Expression of organophosphorus hydrolase OPHC2 in Pichia pastoris: Purification and characterization. Chu, Xiao-Yu,Wu, Ning-Feng,Deng, Min-Jie,Tian, Jian,Yao, Bin,Fan, Yun-Liu.

[2]Fusion expression of bovine lactoferricin in Escherichia coli. Feng, Xing-jun,Wang, Jian-hua,Shan, An-shan,Teng, Da,Yang, Ya-lin,Yao, Yi,Yang, Guan-pin,Shao, Yan-chun,Liu, Shuo,Zhang, Fan.

[3]Purification and identification of cutinases from Colletotrichum kahawae and Colletotrichum gloeosporioides. Chen, Zhenjia,Franco, Catarina F.,Baptista, Ricardo P.,Cabral, Joaquim M. S.,Coelho, Ana V.,Rodrigues, Carlos J., Jr.,Melo, Eduardo P..

[4]Are CuO nanoparticles effects on hemocytes of the marine scallop (Chlamys farreri) caused by particles and/or corresponding released ions?. Sun, Xuemei,Chen, Bijuan,Xia, Bin,Han, Qian,Zhu, Lin,Qu, Kerning,Sun, Xuemei,Chen, Bijuan,Xia, Bin,Zhu, Lin,Qu, Kerning. 2017

[5]Proposal for a unified classification system and nomenclature of lagoviruses. Le Pendu, Jacques,Ruvoen-Clouet, Nathalie,Abrantes, Joana,Lopes, Ana Margarida,Almeida, Tereza,Celio, Alves Paulo,Marques, Sara,Monterroso, Pedro,Silverio, Diogo,Thompson, Gertrudes,Esteves, Pedro,Bertagnoli, Stephane,Guitton, Jean-Sebastien,Marchandeau, Stephane,Almeida, Tereza,Le Gall-Recule, Ghislaine,Alda, Fernando,Celio, Alves Paulo,Goncalves, David,Esteves, Pedro,Celio, Alves Paulo,Barcena, Juan,Blanco, Esther,Burmakina, Galina,Calvete, Carlos,Cavadini, Patrizia,Lavazza, Antonio,Cooke, Brian,Cooke, Brian,Dalton, Kevin,Parra, Francisco,Delibes Mateos, Miguel,Deptula, Wieslaw,Eden, John Sebastian,Eden, John Sebastian,Wang, Fang,Ferreira, Catarina C.,Ferreira, Catarina C.,Ferreira, Paula,Marques, Raquel M.,Ferreira, Paula,Marques, Raquel M.,Foronda, Pilar,Martin-Alonso, Aaron,Gavier-Widen, Dolores,Neimanis, Aleksija,Gavier-Widen, Dolores,Neimanis, Aleksija,Hall, Robin,Strive, Tanja,Hukowska-Szematowicz, Beata,Niedzwiedzka-Rystwej, Paulina,Kerr, Peter,Mah. 2017

[6]Identification of crucial residues of conformational epitopes on VP2 protein of infectious bursal disease virus by phage display. Cui, XL,Nagesha, HS,Holmes, IH.

[7]Genetic polymorphism of ferula mushroom growing on Ferula sinkiangensis. Zhang, JX,Huang, CY,Ng, TB,Wang, HX.

[8]A rapid method for the identification of the soybean cyst nematode Heterodera glycines using duplex PCR. Peng, DL,Moens, M.

[9]Detection of Cryptosporidium Species in Feces from Snakes. Chu, Y. H.,Chen, J. X.,Ai, L.,Cai, Y. C.,Chen, S. H.,Li, J.,He, G. S.. 2012

[10]The microsatellites and minisatellites in the genome of Fenneropenaeus chinensis. Gao, H,Kong, J.

[11]A Ser-Gly substitution in plastid-encoded photosystem II D1 protein is responsible for atrazine resistance in foxtail millet (Setaria italica). Jia, Xiaoping,Yuan, Jincheng,Shi, Yunsu,Song, Yancun,Wang, Guoying,Wang, Tianyu,Li, Yu.

[12]The cloning and sequencing of a cDNA encoding a WD repeat protein in cotton (Gossypium hirsutum L.). HONG Y. DUAN,FU G. LI,XU D. WU,DONG M. MA,MAO WANG,YU X. HOU.

[13]Delimitation of the PSH1(t) gene for rice purple leaf sheath to a 23.5 kb DNA fragment. Wang, Wen-Ying,Ding, Han-Feng,Li, Run-Fang,Liu, Xu,Zhang, Yu,Yao, Fang-Yin,Li, Guang-Xian,Jiang, Ming-Song.

[14]Inheritance of male-sterility and dwarfism in watermelon [Citrullus lanatus (Thunb.) Matsum. and Nakai]. Huang, HX,Zhang, XQ,Wei, ZC,Li, QH,Li, X. 1998

[15]Genomic analysis of an effective lentiviral vaccine-attenuated equine infectious anemia virus vaccine EIAV(FDDV13). Qi, Xu,Su, Zhiqiang,Wang, Xuefeng,Wang, Shuai,Lin, Yuezhi,Jiang, Chenggang,Ma, Jian,Zhao, Liping,Lv, Xiaoling,Shen, Rongxian,Kong, Xiangang,Zhou, Jianhua,Wang, Xuefeng,Wang, Fenglong,Ma, Jian. 2010

[16]Molecular cloning and expression analysis of a hepcidin antimicrobial peptide gene from turbot (Scophthalmus maximus). Chen, Song-Lin,Li, Wei,Meng, Liang,Sha, Zhen-Xia,Wang, Zhi-Jian,Ren, Guo-Cheng. 2007

[17]Analysis of Fitness Predominance for Gaoyou Duck's Double Yolk Egg. Zhang, Tangjie,Chang, Hong,Li, Hui-Fang,Chen, Kuanwei,Zhao, Yonggao,Xuec, Minkai,Zhang, Shengfu. 2011

[18]Dynamic transcriptome and DNA methylome analyses on longissimus dorsi to identify genes underlying intramuscular fat content in pigs. Wang, Yuding,Ma, Cai,Sun, Yi,Kang, Li,Jiang, Yunliang,Wang, Yuding,Li, Yi. 2017

[19]Cloning and overexpression of a Paenibacillus beta-glucanase in Pichia pastoris: Purification and characterization of the recombinant enzyme. Yang, Peilong,Shi, Pengjun,Wang, Yaru,Bai, Yingguo,Meng, Kun,Luo, Huiying,Yuan, Tiezheng,Yao, Bin. 2007

[20]Characterization and molecular cloning of a serine hydroxymethyltransferase 1 (OsSHM1) in rice. Wang, Dekai,Liu, Heqin,Li, Sujuan,Zhai, Guowei,Shao, Jianfeng,Tao, Yuezhi,Wang, Dekai,Liu, Heqin,Li, Sujuan,Zhai, Guowei,Shao, Jianfeng,Tao, Yuezhi. 2015

作者其他论文 更多>>