Detection of DNA ladder during cotyledon senescence in cotton

文献类型： 外文期刊

第一作者： Q.E. XIE

作者： Q.E. XIE;I.D. LIU;S.X. YU;R.F. WANG;Z.X. FAN;Y.G. WANG;F.F. SHEN

作者机构：

关键词： Gossypium hirsutum;apoptosis;DNA fragmentation

期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： The asynchronous programmed cell death (PCD) in different regions of the cotton cotyledon was studied during senescence. We showed that changes in chlorophyll contents do not occur at the same time in different parts of the cotyledon. By using lightmicroscopy (LM) and the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end in situ labelling (TUNEL) methods, the symptoms of cell death were detected in cotyledon margins earlier than in the central part. DNA ladders were detected by gelelectrophoresis in senescent cotyledon margins and in the centre respectively, but not in the whole cotyledons.

分类号： Q94

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