Analysis of differentially expressed genes in response to endogenous cytokinins during cotton leaf senescence

文献类型: 外文期刊

第一作者: P. ZHAO

作者: P. ZHAO;N. ZHANG;Z.J. YIN;Y.D. LIU;F.F. SHEN

作者机构:

关键词: Gossypium hirsutum;metabolic pathways;Solexa sequencing;TIGR database;transgenic plants

期刊名称:BIOLOGIA PLANTARUM ( 影响因子:1.747; 五年影响因子:2.146 )

ISSN: 0006-3134

年卷期: 2013 年 57 卷 3 期

页码:

收录情况: SCI

摘要: Cytokinins have been implicated in delaying leaf senescence. We previously generated transgenic cotton (Gossypium hirsutum L.) plants that harbor the Agrobacterium isopentenyl transferase gene (ipt) directed by a proteinase gene promoter. Here, we report that mRNAs were isolated from ipt cotton leaves and azygous leaves and were subsequently sequenced using Illumina Solexa technology. The sequence tags were searched against the TIGR database and the related gene expression profiles were compared resulting in the identification of 1 218 differentially expressed genes (DEGs): 719 up-regulated and 499 down-regulated. Analyzing the DEGs in the ipt cotton leaves showed that these genes belonged to four pathways: flavone biosynthesis, arginine and proline metabolism, glyoxylate and dicarboxylate metabolism, and RNA degradation. These pathways increased the activities of antioxidants, inhibited the effect of ethylene, and prevented degradation of macromolecules during senescence. The expression patterns of 17 genes were evaluated by real-time PCR and results were in agreement with the patterns of sequencing analysis. The identification of the DEGs may help us to understand a role of cytokinins in leaf senescence.

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