Improved salt tolerance and delayed leaf senescence in transgenic cotton expressing the Agrobacterium IPT gene

文献类型： 外文期刊

第一作者： Y.D. Liu;;Z.J. Yin

作者： Y.D. Liu;Z.J. Yin;J.W. YU;J. LI;H.L. WEI;X.L. HAN;F.F. SHEN

作者机构：

关键词： Gossypium hirsutum;isopentenyl transferase;cytokinins;NaCl;senescence-specific promoter

期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The manipulation of cytokinin contents via Agrobacterium-mediated transformation is an efficient tool for delaying leaf senescence and improving the resistance to environmental stresses. In the present study, cotton transformants harbouring the Agrobacterium tumefaciens isopentenyl transferase (IPT) gene under the control of the promoter of Gossypium hirsutum cysteine proteinase (Ghcysp) were generated. PCR and Southern blot analysis indicated that the foreign DNA fragment was successfully integrated into the cotton genome. The chlorophyll and cytokinin contents, and ROS-scavenging enzymatic activities were significantly increased in transgenic cotton lines, which resulted in a significant delay in leaf senescence. The growth characteristics of transgenic cotton lines resembled the non-transgenic lines except delaying premature senescence and the lint yield and fiber quality of transgenic lines were improved. In addition, the transgenic lines had higher biomasses, IPT transcripts, and endogenous cytokinin contents compared with those of non-transgenic lines under 200 mM NaCl stress.

分类号： Q94

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