In vitro assay for 2,4-D resistance in transgenic cotton

文献类型： 外文期刊

第一作者： BAO-HONG ZHANG

作者： BAO-HONG ZHANG;HONG-MEI WANG;FANG LIU;YUN-HAI LI;ZHENG-DE LIU

作者机构：

关键词： Gossypium hirsutum L;transgenic cotton;in vitro assay;kanamycin;2;4-D

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT （ 影响因子：2.252； 五年影响因子：2.139 ）

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收录情况： SCI

摘要： 2,4-Dichlorophenoxyacetio acid (2,4-D) resistant plants of transgenic cotton (Gossypium hirsutum L.) were produced using Agrobacterium tumefaciens containing a plasmid carrying the neornycin phosphotransferase II (npt II) and 2,4-D monooxygenase (tfd A) genes. An in vitro assay was performed to determine the sensitivity of seed germination, and the growth of seedlings of transgenic and non-transgenic cotton to various concentrations of kanamycin and 2,4-D. The results indicated that kanamycin caused the cotyledons of non-transgenic plants to turn white, but transgenic plants grew normally. Seed germination and seedling growth of non-transgenic plants were strongly inhibited by 2,4-D, but only slightly for transgenic plants. Transgenic plants and non-transgenic plants can be clearly distinguished by the use of 2 mg l~(-1) 2,4-D in seed germination medium. There was a high correlation between the response of seed germination and the growth of seedlings to kanamycin or 2,4-D, based on the germination ratio, albino ratio, dry weight or fresh weight. On this basis, we developed a rapid method for identifying transgenic plants that has been verified in the field. These findings will allow identification of cotton transformants at an early stage of plant development, saving time and improving cultivars containing the 2,4-D resistance trait.

分类号： Q94

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