Effects of kanamycin on tissue culture and somatic embryogenesis in cotton

文献类型： 外文期刊

第一作者： Bao-Hong Zhang

作者： Bao-Hong Zhang;Fang Liu;Zhi-Hong Liu;Hong-Mei Wang;Chang-Bing Yao

作者机构：

关键词： Gossypium hirsutum l;Kanamycin;Somatic embryogenesis;Tissue;Gossypium-hirsutum-l;Transgenic cotton;Particle bombardment;Plant-regeneration;Aminoglycoside antibiotics;Agrobacterium-tumefaciens;Genetic-transformation;Nucleotide-sequence;Resistance;Expression

期刊名称：PLANT GROWTH REGULATION （ 影响因子：3.412； 五年影响因子：3.691 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The aminoglycoside antibiotic kanamycin was evaluated for its effects on callus initiation from hypocotyl and cotyledon explants, proliferation of non-embryogenic and embryogenic calli, initiation and development of somatic embryos in cotton (Gossypium hirsutum L.). On this basis, the potential use of kanamycin as a selective agent in genetic transformation with the neomycin phosphotransferase II gene as the selective marker gene was evaluated. Cotton cotyledon and hypocotyl explants, and embryogenic calluses were highly sensitive to kanamycin. Kanamycin at 10 mg/L or higher concentrations reduced callus formation, with complete inhibition at 60 mg/L. Kanamycin inhibited embryogenic callus growth and proliferation, as well as the initiation and development of cotton somatic embryos. The sensitivity of embryogenic callus and somatic embryos to kanamycin was different during the initiation and development stages. Kanamycin was considered as a suitable selective agent for transformed callus formation and growth of non-embryogenic callus. Forty to sixty mg/L was the optimal kanamycin concentration for the induction and proliferation of transformed callus. The concentration of kanamycin must be increased (from 50 to 200 mg/L) for the selection of transformation embryogenic callus and somatic embryos. A scheme for selection of transgenic cotton plants when kanamycin is used as the selection agent is discussed.

分类号： S

  • 相关文献

[1]In vitro assay for 2,4-D resistance in transgenic cotton. BAO-HONG ZHANG,HONG-MEI WANG,FANG LIU,YUN-HAI LI,ZHENG-DE LIU.

[2]Improvement of Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizing selection regimes and utilizing cryopreserved cell suspensions. Li, P,Hanania, U,Sahar, N,Mawassi, M,Gafny, R,Sela, I,Tanne, E,Perl, A.

[3]Efficient somatic embryogenesis in sugar beet (Beta vulgaris L.) breeding lines. Zhang, Chun-Lai,Chen, Dong-Fang,Kubalakova, Marie,Zhang, Jian,Scott, Nigel W.,Elliott, Malcolm C.,Slater, Adrian,Zhang, Chun-Lai,Chen, Dong-Fang,Kubalakova, Marie,Zhang, Jian.

[4]THE EFFECTS OF 2IP AND 2,4-D ON RICE CALLI DIFFERENTIATION. Zhu, YX,Ouyang, WJ,Li, Y,Chen, ZL.

[5]Somatic embryogenesis from thin epidermal layers in sunflower and chromosomal regions controlling the response. Huang, XianQun,Nabipour, Alireza,Gentzbittel, Laurent,Sarrafi, Ahmad.

[6]Development of insect-resistant transgenic cotton with chimeric TVip3A*accumulating in chloroplasts. Wu, Jiahe,Zhang, Xiangrong,Tian, Yingchuan,Luo, Xiaoli,Shi, Yuejing. 2011

[7]Recent progress in cotton biotechnology and genetic engineering in China. Bao-Hong Zhang,Fang Liu,Chang Bing Yao,Kun-Bo Wang.

[8]Cloning and characterization of a novel Cry1A toxin from Bacillus thuringiensis with high toxicity to the Asian corn borer and other lepidopteran insects. Liang, Gemei,Li, Haitao,He, Kanglai,Song, Fuping,Feng, Xue,Zhang, Jie,Crickmore, Neil,Huang, Dafang.

[9]Engineered Bacillus thuringiensis GO33A with broad insecticidal activity against lepidopteran and coleopteran pests. Wang, Guangjun,Zhang, Jie,Song, Fuping,Wu, Jun,Feng, Shuliang,Huang, Dafang.

[10]A Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus dabanensis D-8(T): cloning and molecular characterization. Yang, LF,Jiang, JQ,Zhao, BS,Zhang, B,Feng, DQ,Lu, WD,Wang, L,Yang, SS.

[11]Molecular cloning and characterization of a mannose-binding lectin gene from Crinum asiaticum. Pang, YZ,Liao, ZH,Zhang, L,Sun, XF,Lu, YQ,Wang, SG,Tang, KX.

[12]Mining, identification and function analysis of microRNAs and target genes in peanut (Arachis hypogaea L.). Zhang, Tingting,Hu, Shuhao,Yan, Caixia,Li, Chunjuan,Zhao, Xiaobo,Shan, Shihua,Hu, Shuhao,Wan, Shubo.

[13]A delta-endotoxin encoded in Pseudomonas fluorescens displays a high degree of insecticidal activity. Peng, R,Xiong, A,Li, X,Fuan, H,Yao, Q.

[14]Large-scale production of enhancer trapping lines for rice functional genomics. Yang, YZ,Peng, H,Huang, HM,Wu, JX,Ha, SR,Huang, DF,Lu, TG.

[15]A Visual Gel-Enzyme-Linked Immunosorbent Assay for Simultaneous Detection of Gentamicin and Kanamycin in Milk. Xu Fei,Liu Mao-Lin,Liu Yi-Ming,Li Xiu-Bo,Li Jing-Ya,Zhou Jie,Ding Shuang-Yang,Wang Jian-Fen. 2015

[16]Aptamer-functionalized magnetic nanoparticles for simultaneous fluorometric determination of oxytetracycline and kanamycin. Liu, Changbin,Lu, Chunxia,Tang, Zonggui,Chen, Xia,Wang, Guohong,Sun, Fengxia.

[17]Aptamer based fluorometric determination of kanamycin using double-stranded DNA and carbon nanotubes. Liao, Qie Gen,Wei, Ben Hua,Luo, Lin Guang.

[18]Transgenic fertile japonica rice plants expressing a modified crylA(b) gene resistant to yellow stem borer. Wu, C,Fan, Y,Zhang, C,Oliva, N,Datta, SK. 1997

[19]Creation of transgenic bananas expressing human lysozyme gene for Panama wilt resistance. Pei, XW,Chen, SK,Wen, RM,Ye, S,Huang, JQ,Zhang, YQ,Wang, BS,Wang, ZX,Jia, SR. 2005

[20]Efficient regeneration and genetic transformation platform applicable to five Musa varieties. Liu, Juhua,Gao, Pengzhao,Zhang, Jing,Jia, Caihong,Zhang, Jianbin,Hu, Wei,Xu, Biyu,Jin, Zhiqiang,Sun, Peiguang,Wang, Jiashui,Jin, Zhiqiang,Sun, Xiuxiu. 2017

作者其他论文 更多>>

微信小程序