Cloning of metallothionein genes from Arachis hypogaea and characterization of AhMT2a

文献类型： 外文期刊

第一作者： Quan, X. Q.

作者： Quan, X. Q.;Shan, L.;Bi, Y. P.

作者机构：

关键词： Arachis hypogaea;metallothionein;reactive oxygen species

期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：1.481； 五年影响因子：1.608 ）

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年卷期：

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收录情况： SCI

摘要： Seven cDNA sequences putatively encoding metallothionein (MT) proteins were isolated from peanut (Arachis hypogaea L.). We analyzed the sequences of the deduced amino acids and characterized one of these genes, AhMT2a. Northern blot analysis indicates that the accumulation of AhMT2a mRNA was found in stems, young roots, and young leaves, and AhMT2a mRNA levels were up-regulated by cadmium, copper, ABA, HO, and heat shock. The metal-binding properties of the recombinant protein expressed in E. coli was investigated, and the results show that bacteria harboring AhMT2a exhibited increased tolerance to cadmium, copper, and lead, and the metal accumulation was also higher than the controls. Taken together, our results suggest that the AhMT2a protein might function in both detoxification of heavy metals and scavenging of reactive oxygen species in peanut.

分类号： Q94

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