Protective immune responses in guinea pigs and swine induced by a suicidal DNA vaccine of the capsid gene of swine vesicular disease virus

文献类型： 外文期刊

第一作者： Sun, Shi-Qi

作者： Sun, Shi-Qi;Liu, Xiang-Tao;Guo, Hui-Chen;Yin, Shuang-Hui;Shang, You-Jun;Feng, Xia;Liu, Zai-Xin;Xie, Cling-Ge

作者机构：

关键词： MOUTH-DISEASE;CELL-DEATH;CDNA-CLONE;IN-VITRO;EXPRESSION;VECTORS;REPLICON;PROTEIN;FOOT;IDENTIFICATION

期刊名称：JOURNAL OF GENERAL VIROLOGY （ 影响因子：3.891； 五年影响因子：3.719 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A suicidal DNA vaccine based on a Semliki Forest virus (SFV) replicon was evaluated for the development of a vaccine against swine vesicular disease virus (SVDV). The 1BCD gene of SVDV was cloned and inserted into pSCA1, an SFV DNA-based replicon vector. The resultant plasmid, pSCA/1BCD, was transfected into BHK-21 cells and the antigenicity of the expressed protein was confirmed using an indirect immunofluorescence assay. Immunogenicity was studied in guinea pigs and swine. Animals were injected intramuscularly three times with pSCA/1BCD at regular intervals. Anti-SVDV antibodies were detected by ELISA, the lymphocyte proliferation response was tested by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide method and neutralizing antibodies were measured by microneutralization tests. The data showed that SVDV-specific antibodies, neutralizing antibodies and lymphocyte proliferation were induced in both guinea pigs and swine. Furthermore, after three successive vaccinations with pSCA/1BCD, half of the pigs were protected against challenge with SVDV. These results should encourage further work towards the development of a DNA vaccine against SVDV.

分类号： R37

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