SEQUENCE ANALYSIS OF WHEAT AND OAT FUROVIRUS CAPSID PROTEIN GENES SUGGESTS THAT OAT GOLDEN STRIPE VIRUS IS A STRAIN OF SOIL-BORNE WHEAT MOSAIC VIRUS

文献类型： 外文期刊

第一作者： Chen, JP

作者： Chen, JP;Shi, NN;Michael, T;Wilson, A;Antoniw, JF;MacFarlane, SA;Adams, MJ

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关键词： Soil borne wheat mosaic virus;Oat golden stripe virus;Furoviruses

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

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收录情况： SCI

摘要： In northern blots, cDNA probes prepared to soil-borne wheat mosaic virus (SBWMV) RNA-1 and RNA-2 hybridized to RNA-1 and RNA-2, respectively, from a UK isolate of oat golden stripe virus (OGSV), as well as to their homologous RNAs. RT-PCR was used to amplify, clone and sequence a region of about 750 nucleotides spanning the capsid protein gene and part of the readthrough protein on RNA-2 from OGSV, a French isolate of SBWMV and two stable deletion mutants (Lab1 and Okl-7) of SBWMV isolates from Nebraska and Oklahoma respectively. There was very high (96.7-99.1%) nucleotide homology between all these sequences and the wild-type SBWMV sequences from Nebraska and Oklahoma. OGSV was more similar to SBWMV from France and Nebraska than were any of the isolates to SBWMV from Oklahoma. Of the few differences in the deduced amino acid sequences of the capsid proteins from the different isolates, OGSV differed from all SBWMV isolates only in one amino acid (isoleucine for valine at position 88). The high degree of similarity suggests that OGSV may best be classified as an oat strain of SBWMV.

分类号： R37

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