Structural Characteristic and Genetic Expression of nodulin-like Gene and Its Promoter in Cotton

文献类型： 外文期刊

第一作者： Ren, MZ

作者： Ren, MZ;Chen, QJ;Zhang, R;Guo, SD

作者机构：

关键词： nodulin-like gene;promoter;structural characteristic;genetic expression;cotton

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Full-length DNA, cDNA and promoter sequences of nodulin-like gene have been isolated and characterized by isocaudamer inverse PCR (Ⅱ -PCR) and rapid isolating cDNA 5' unknown sequence and promoter (RICUP) methods from cotton. Sequence analysis indicated that nodulin-like gene was 2353 bp in size, including five introns and six exons. Its full-length cDNA was 1480 bp, containing an 1125-bp ORF structure, which encoded a 40.23-kD polypeptide with predicted isoelectric point of 8.433. A check against nr database in GenBank indicated that no DNA sequence was found to share homology with it Searched in EST database, a 733-bp EST sequence of Gossypium arboreum L. (GenBank asscession number BF271235} was found to share 92% homology with it Promoter of nodulin-like gene was 1969 bp in length with several typical sequences, such as initiator, TATA box, CAAT-like box and AT-rich sequence. Southern blotting analysis suggested that one copy of nodulin-like gene was integrated into cotton genome. Northern blotting analysis showed that nodulin-like gene expressed preferentialy in fiber and reproductive organs of cotton, such as bud, flower and boll. Our work provides researchers with candidate genes to improve agronmic traits of cotton and also provide efficient expression elements for problem solving in transgenic Bt cotton, namely resistance to bollworm declining dramatically at late developmental stage of cotton.

分类号： Q94

  • 相关文献

[1]Molecular cloning and analysis of a receptor-like promoter of Gbvdr3 gene in sea island cotton. Zhang, B. -J.,Chen, Q. -Z.,Tang, N.,Wang, L. -K.,Zhang, B. -J.,Zhang, H. -P.,Wang, R. -F.,Zhang, B. -L.. 2016

[2]Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton. Shang, Hai-Hong,Liu, Chuan-Liang,Zhang, Chao-Jun,Li, Feng-Lian,Hong, Wei-Dong,Li, Fu-Guang.

[3]Characterization of the promoter of phosphate transporter TaPHT1.2 differentially expressed in wheat varieties. Miao, Jun,Sun, Jinghan,Liu, Dongcheng,Li, Bin,Zhang, Aimin,Li, Zhensheng,Tong, Yiping,Miao, Jun. 2009

[4]Regulatory mutations in the A2M gene are involved in the mastitis susceptibility in dairy cows. Wang, X. G.,Huang, J. M.,Feng, M. Y.,Ju, Z. H.,Wang, C. F.,Zhong, J. F.,Yang, G. W.,Yuan, J. D.. 2014

[5]Variants and Gene Expression of the TLR2 Gene and Susceptibility to Mastitis in Cattle. Huang, Jinming,Liu, Li,Wang, Hongmei,Zhang, Cuixia,Ju, Zhihua,Wang, Changfa,Zhong, Jifeng.

[6]Function identification of bovine Nramp1 promoter and intron 1. Hao, Linlin,Zhang, Libo,Liu, Songcai,Li, Mingtang,Zhong, Jifeng,Wang, Nan. 2011

[7]Identification and genetic effect of haplotypes in the promoter region of porcine myostatin gene. Liu, D.,Xu, Q.,Zang, L.,Liang, S.,Jiang, Y.,Wu, Y.,Wei, S.. 2011

[8]Specific Expression of Maize SBEIIb Promoter Mediated by Different Promoter Region in Transgenic Tobacco Plants. Sun Cui-xia,Li Meng,Wang Xiao-peng,Zhang Guo-dong,Tian Yan-chen,Wang Ze-li,Han Jing. 2009

[9]Identification and preliminary analysis of a new PCP promoter from Brassica rapa ssp chinensis. Zhang, Qiang,Cao, Jiashu,Zhang, Qiang,Liu, Huizhi. 2008

[10]p53 and NF kappa B regulate microRNA-34c expression in porcine ovarian granulosa cells. Xu Yuan,Xiao Guang,Zhang Zhe,Chen Zan-mou,Zhang Hao,Li Jia-qi,Zhang Ai-ling. 2016

[11]Expression regulation of a xylanase inhibitor gene riceXIP in rice (Oryza sativa L.). Zhan, Yihua,Sun, Xiangyu,Xu, Ying,Huang, Yingying,Jiang, Dean,Weng, Xiaoyan,Sun, Renjie,Hou, Chunxiao,Hou, Chunxiao. 2017

[12]Promoter Characterization and Expression Pattern Analysis of Porcine TCAP Gene. Qiao, Mu,Wu, Huayu,Huang, Jingshu,Peng, Xianwen,Liu, Guisheng,Feng, Zheng,Mei, Shuqi. 2012

[13]The SOD Gene Family in Tomato: Identification, Phylogenetic Relationships, and Expression Patterns. Feng, Kun,Zheng, Qingsong,Feng, Kun,Yu, Jiahong,Cheng, Yuan,Ruan, Meiying,Wang, Rongqing,Ye, Qingjing,Zhou, Guozhi,Li, Zhimiao,Yao, Zhuping,Yang, Yuejian,Wan, Hongjian,Yu, Jiahong. 2016

[14]Isolation of the endosperm-specific LPAAT gene promoter from coconut (Cocos nucifera L.) and its functional analysis in transgenic rice plants. Zheng, Yusheng,Wang, Zhekui,Li, Dongdong,Xu, Li,Zhou, Peng,Ye, Rongjian,Lin, Yongjun,Ye, Rongjian,Lin, Yongjun. 2010

[15]Functional analysis of the larval serum protein gene promoter from silkworm, Bombyx mori.. Tang, SM,Yi, YZ,Shen, XJ,Zhang, ZF,Li, YR,He, JL. 2003

[16]Identification of novel transcripts from the porcine MYL1 gene and initial characterization of its promoters. Li, Jiaqi,Wang, Liangliang,Zhang, Hao,Chen, Songling,Mei, Yingjie,Wang, Chong,Ling, Fei,Du, HongLi,Fang, Wei,Chen, Yaosheng,Liu, Xiaohui. 2010

[17]Isolation of a maize beta-glucosidase gene promoter and characterization of its activity in transgenic tobacco. Gu, Riliang,Zhao, Li,Zhang, Ying,Chen, Xiaoping,Bao, Juan,Zhao, Jinfeng,Wang, Zhangying,Fu, Junjie,Liu, Tingsong,Wang, Jianhua,Wang, Guoying. 2006

[18]Homologous region 3 from Bombyx mori nucleopolyhedrovirus enhancing the transcriptional activity of heat shock cognate 70-4 promoter from Bombyx mori and Bombyx mandarina in vitro and in vivo. Tang, SM,Zhao, QL,Yi, YZ,Zhang, ZF,Li, YR. 2005

[19]A Novel Soybean Intrinsic Protein Gene, GmTIP2;3, Involved in Responding to Osmotic Stress. Zhang, Dayong,He, Xiaolan,Xu, Zhaolong,Xu, Ling,Wei, Peipei,Huang, Yihong,Brestic, Marian,Ma, Hongxiang,Shao, Hongbo,Tong, Jinfeng,Brestic, Marian,Shao, Hongbo. 2016

[20]Isolation of DNA-Methyltransferase Genes and Their Promoter Sequences from Strawberry (Fragaria x ananassa Duch.). Chang, Linlin,Zhang, Yuntao,Chang, Linlin,Zhang, Zhihong,Li, He,Yang, Yue,Dai, Hongyan. 2011

作者其他论文 更多>>

微信小程序