A generic real-time TaqMan assay for specific detection of lapinized Chinese vaccines against classical swine fever

文献类型： 外文期刊

第一作者： Liu, Lihong

作者： Liu, Lihong;Xia, Hongyan;Belak, Sandor;Widen, Frederik;Liu, Lihong;Xia, Hongyan;Belak, Sandor;Widen, Frederik;Xia, Hongyan;Belak, Sandor;Everett, Helen;Sosan, Olubukola;Crooke, Helen;Meindl-Boehmer, Alexandra;Moennig, Volker;Meindl-Boehmer, Alexandra;Moennig, Volker;Qiu, Hua-Ji

作者机构：

关键词： gene;lapinized Chinese vaccine: immunologic-drug;immunostimulant-drug;vaccine;genome

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Classical swine fever (CSF) is a highly contagious disease, causing severe economic losses in the pig industry worldwide. Vaccination of pigs with lapinized Chinese vaccines is still practised in some regions of the world, where the virus is enzootic, in order to prevent and control the disease. However, a single real-time assay that can detect all lapinized Chinese vaccines used widely, namely, Lapinized Philippines Coronel (LPC), Hog Cholera Lapinized virus (HCLV) and the Riems C-strain is still lacking. This study describes a real-time RT-PCR assay, targeting the N-pro gene region, for specific detection of these lapinized vaccine strains. The assay is highly sensitive, with a detection limit of 10 genome copies per reaction for HCLV and Riems C-strain and highly specific, as more than 100 strains of wild type CSFV representing all major genotypes were not detected. The assay is also highly repeatable: the coefficient of variation of Ct values in three runs was 2.77% for the detection of 10 copies of the vaccine viral RNA. This study provides a potentially useful tool for specific detection of the lapinized Chinese vaccines, HCLV and C-strain, and the differentiation of these vaccines from wild type CSFV

分类号： R37

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