Cloning and characterization of a beta-1,3-glucan-binding protein from shrimp Fenneropenaeus chinensis

文献类型： 外文期刊

第一作者： Lai, Xiaofang

作者： Lai, Xiaofang;Kong, Jie;Wang, Qingyin;Wang, Weiji;Meng, Xianhong;Lai, Xiaofang;Lai, Xiaofang

作者机构：

关键词： cDNA: complementary DNA;open reading frame;beta-1;3-D-glucan binding protein-HDL: expression;RGD motif;glucanase-like motifSequence DataAY249;GU461662: GenBank;EMBL;DDJB;nucleotide sequence;immune response;shrimp farming

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： The beta-1,3-d-glucan binding protein (beta GBP), one kind of the pattern recognition proteins (PRPs), was cloned and characterized from the Chinese Shrimp Fenneropenaeus chinensis, and named as Fc beta GBP-HDL. The results indicated that the full length cDNA of 6713 bp had an open reading frame encoded a polypeptide of 2139 amino acids with two glucanase-like motifs and one RGD motif, while without signal peptide. The calculated molecular mass of mature protein was 240.7 kDa and theoretical pI was 5.95. Sequence comparison of the deduced amino acid sequence of Fc beta GBP-HDL showed varied identity of 88, 54 and 53% with those of Litopenaeus vannamei beta GBP-HDL, Pacifastacus leniusculus beta GBP, and Pontastacus leptodactylus beta GBP, respectively. qRT-PCR analysis indicated that Fc beta GBP-HDL was expressed in intestines, hepatopancreas, muscle, gill and hemocytes, and its profile was modified post WSSV challenge. The differential expressions of Fc beta GBP-HDL in different tissues post WSSV challenge suggested that Fc beta GBP-HDL might play an important role in shrimp immune and perform differently in different tissues. These data would be helpful to better understand the WSSV-resistance mechanism of farming shrimp.

分类号： Q7

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