Identification of the heat shock protein 70 (HLHsp70) in Haemaphysalis longicornis

文献类型： 外文期刊

第一作者： Tian, Zhancheng

作者： Tian, Zhancheng;Liu, Guangyuan;Zhang, Liyan;Yin, Hong;Xie, Junren;Zhang, Ping;Luo, Jin;Wang, Hui

作者机构：

关键词： amino acid residue;antiserum;glucose-regulated protein;heat shock protein 70: Hsp70;expression;endoplasmic reticulum-localized protein;cDNA library;molecular weight;phylogenetic relationship;oviposition;blood-feeding;isoelectronic point

期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.738； 五年影响因子：2.951 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A Haemaphysalis longicornis heat shock protein 70 (HLHsp70) was identified from a cDNA library synthesized from tick eggs. The HLHsp70 cDNA is 2311 bp in length and encodes 661 amino acid residues with the predicted molecular weight of 72.5 kDa and an isoelectronic point (pl) of 5.2. It also contains the highly conserved functional motifs of the Hsp70 family and a specific endoplasmic reticulum (ER) retention signal "KDEL" that is common among ER-localized proteins. The HLHsp70 exhibits 90% amino acid identity to the putative Hsp70 of Ixodes scapularis, and 85% to Gallus gallus 78 kDa glucose-regulated protein precursor. Real time RT-PCR analysis showed that the expression levels of the Hsp70 in ovaries and salivary glands were significantly higher than in other tested tissues in partially fed females. Although the expression level of the HLHsp70 was constantly low in unfed ticks, it was significantly induced by blood-feeding. Further, the expression was positively correlated to the temperature (4-37 degrees C, tested). Western blot analysis showed that the rabbit antiserum against the recombinant HLHsp70 protein (rHLHSP70) recognized bands of approximately 100, 72, and 28 kDa from egg lysates, as well as a 72 kDa fragment in protein extracts from partially fed larvae. Immunization of rabbits with the rHLHSP70 did not result in a statistically significant reduction of female tick engorgement and oviposition. These results suggest that although HLHSP70 plays a role in the physiological activities of ticks, as a constitutive protein it was not suitable for selection as a candidate vaccine antigen against ticks

分类号： S85

  • 相关文献

[1]Characterization of microRNAs from sheep (Ovis aries) using computational and experimental analyses. Sheng, Xihui,Wei, Caihong,Liu, Tao,Zhang, Li,Du, Lixin,Sheng, Xihui,Song, Xuemei,Yu, Yan,Niu, Lili,Li, Shangang,Li, Hongbin.

[2]Construction and Evaluation of Normalized cDNA Libraries Enriched with Full-Length Sequences for Rapid Discovery of New Genes from Sisal (Agave sisalana Perr.) Different Developmental Stages. Zhou, Wen-Zhao,Zhang, Yan-Mei,Lu, Jun-Ying,Li, Jun-Feng. 2012

[3]Differential expression of expressed sequence tags in alfalfa roots under aluminum stress. Fan, Fu,Li, Xin-Wei,Wu, Yue-Ming,Xia, Zhuo-Sheng,Li, Jin-Jun,Zhu, Wen,Liu, Jin-Xin,Li, Jin-Jun.

[4]Production of antisera against porcine haptoglobin: Potential for distinguishing haptoglobin subunits. Yang, Yongqian,An, Tongqing,Liu, Fei,Yuan, Zhonghua,Peng, Jinmei,Wu, Yuquan,Meng, Zhenxiang,Tian, Zhijun,Yang, Yongqian,Wu, Jiang,Zhang, Deli.

[5]Integration of lncRNA-miRNA-mRNA reveals novel insights into oviposition regulation in honey bees. Chen, Xiao,Chen, Chao,Shi, Wei,Liu, Zhiguang,Wang, Huihua,Guo, Haikun,Ma, Ce,Lu, Qian. 2017

[6]Fitness of Encarsia sophia (Hymenoptera: Aphelinidae) parasitizing Trialeurodes vaporariorum and Bemisia tabaci (Hemiptera: Aleyrodidae). Luo, Chen,Liu, Tong-Xian,Luo, Chen. 2011

[7]Variation of pre-ovipositional period in the brown planthopper, Nilaparvata lugens, collected in tropical, subtropical and temperate Asia. Wada, T.,Ito, K.,Takahashi, A.,Tang, J.. 2007

[8]Host Status of Wheat and Corn for Chilo suppressalis (Lepidoptera: Crambidae). Hao, Lixia,Han, Yongqiang,Hao, Lixia,Liao, Xiaolan.

[9]The immunomodulatory protein RH36 is relating to blood-feeding success and oviposition in hard ticks. Wang, Fangfang,Lu, Xiaojuan,Guo, Fengxun,Gong, Haiyan,Zhang, Houshuang,Zhou, Yongzhi,Cao, Jie,Zhou, Jinlin,Zhou, Jinlin.

[10]Mycangial fungus benefits the development of a leaf-rolling weevil, Euops chinesis. Ding, Jianqing,Li, Xiaoqiong,Guo, Wenfeng.

[11]Electrophysiological Responses and Reproductive Behavior of Fall Webworm Moths (Hyphantria cunea Drury) are Influenced by Volatile Compounds from Its Mulberry Host (Morus alba L.). Tang, Rui,Zhang, Zhong-Ning,Tang, Rui,Zhang, Feng,Tang, Rui,Zhang, Feng. 2016

[12]Effects of mating on ovarian development and oviposition of Apolygus lucorum. Yuan, Wei,Li, Yunhe,Wu, Kongming,Zhao, Xincheng. 2017

[13]Emergence, Mating and Oviposition Behavior of the Chinese Population in Pink Bollworm, Pectinophora gossypiella (Lepidoptera: Gelechiidae). Wi Huai-heng,Wu Kong-ming,Wi Huai-heng,Huang Min-song,Wan Peng,Wyckhuys, Kris A. G.. 2013

[14]EFFECT OF TEMPERATURE AND SUPPLEMENTARY NUTRITION ON THE DEVELOPMENT, LONGEVITY AND OVIPOSITION OF CONOPOMORPHA SINENSIS (LEPIDOPTERA: GRACILLARIIDAE). Li, Pengyan,Chen, Bingxu,Dong, Yizhi,Xu, Shu,Chen, Kaige. 2013

[15]Mating frequency positively associates with fitness in Ophraella communa. Zheng, Hai-Yan,Guo, Jian-Ying,Wan, Fang-Hao,Rasmann, Sergio,Zheng, Hai-Yan,Wang, Jian-Guo,Watson, Alan.

[16]Electrophysiological and Behavioral Responses of Chrysopa phyllochroma (Neuroptera: Chrysopidae) to Plant Volatiles. Xu, Xiuxiu,Cai, Xiaoming,Bian, Lei,Luo, Zongxiu,Xin, Zhaojun,Chen, Zongmao.

[17]The characteristics of oviposition and hormonal and gene regulation of ovarian follicle development in Magang geese. Qin, Qingming,Guo, Rihong,Lei, Mingming,Ying, Shijia,Shi, Zhendan,Sun, Aidong. 2013

[18]Development of Microplitis bicoloratus on Spodoptera litura and implications for biological control. Luo, Kaijun,Trumble, John T.,Pang, Yi.

[19]Comparison of the colony development of two native bumblebee species Bombus ignitus and Bombus lucorum as candidates for commercial pollination in China. Wu, Jie,Peng, Wenjun,An, Jiandong,Huang, Jiaxing,Li, Jilian,Cai, Wanzhi. 2008

[20]Feeding, oviposition and survival of Liriomyza trifolii (Diptera: Agromyzidae) on Bt and non-Bt cottons. Liu, T. -X.,Lei, Z.,Greenberg, S. M.. 2009

作者其他论文 更多>>

微信小程序